Anti-Mouse CD32/CD16 – Purified In vivo GOLD™ Functional Grade

Pricing & Details

Product No.C381
Clone
2.4G2
Protein
CD32/CD16
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Fcγ R III/II, Ly-17
Isotype
Rat
IgG2b
Applications
CODEX®
,
FA
,
FC
,
IP
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C381-1.0 mg
1.0 mg
$95.00
In stock
Max:
Min: 1
Step: 1
C381-5.0 mg
5.0 mg
$298.00
In stock
Max:
Min: 1
Step: 1
C381-25 mg
25 mg
$775.00
In stock
Max:
Min: 1
Step: 1
C381-50 mg
50 mg
$1,275.00
In stock
Max:
Min: 1
Step: 1
C381-100 mg
100 mg
$1,998.00
In stock
Max:
Min: 1
Step: 1

Data

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Sorted pre-B cells
Product Concentration
≥ 2.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Applications and Recommended Usage
Blocking: Rat Anti-Mouse CD32/CD16 Purified Fc UltraBlock™ may be used for blocking Fc receptor-mediated binding by directly labeled antibodies to reduce background staining in flow cytometry.
1. Incubate cells with Anti-Mouse CD32/CD16 Purified Fc UltraBlock™ (≤1 µg per 1 x 106 cells in 100 µl total staining volume) at 4°C for 5 minutes.
2. Add the conjugated primary antibody directly to the preincubated cells. Anti-Mouse CD32/CD16 Purified Fc UltraBlock™ does not need to be washed away prior to cell staining.
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this 2.4G2 antibody for staining cells in flow cytometry is ≤ 1 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this 2.4G2 antibody for use in western blotting is 1-10 μg/ml.
Other Applications Reported In Literature ?
CODEX®
Additional Reported Applications For Relevant Conjugates ?
B
IP
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 2.4G2 recognizes the FcγIII and FcγII receptors.
Antigen Distribution
These receptors are present on B cells, monocyte/macrophages, NK cells, neutrophils, mast cells and dendritic cells.
Background
CD16 is expressed in two forms: CD16a and CD16b. CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. CD16b (FcγRIIIB) is a 48 kD GPI-anchored protein whose extracellular domain is over 95% homologous to that of CD16a. CD16 regulates both phagocytosis and antibody-dependent cell-mediated cytotoxicity. It has been reported that CD16 is involved in Natural Killer Cell activation and plays a role in signal transduction. The receptors, CD32 (FcγRIII) and CD16 (FcγRII), are 40-60 kD and bind antibody-antigen immune complexes and mediate adaptive immune responses.

Antigen Details

Protein
Ligand/Receptor
IgG
Function
Low affinity receptors for IgG
PubMed
NCBI Gene Bank ID

References & Citations

1. Titas, J. A. et al. (1982) J. Immunol. 133:556 2. Rodewald, H. et al. (1992) Cell 69:139
Flow Cytometry
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.