Anti-Mouse CD32/CD16 – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Anti-Mouse CD32/CD16 – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Product No.: C524

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Clone
2.4G2
Target
CD32/CD16
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Fcγ R III/II, Ly-17
Isotype
IgG2b
Applications
IHC FF
,
PhenoCycler®

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Product Size
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CODEX® Details

Validation Notes
Clone 2.4G2 has been validated for use on CODEX® using mouse spleen fresh frozen (FF) tissue.
Tissue Screened
Spleen
Tissue Preparation
FF (Fresh Frozen)

Antibody and Reporter Details

Reactivity Species
Mouse
Host Species
Rat
Concentration
0.5 mg/ml
Immunogen
Sorted pre-B cells
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX® This CD32/CD16 (Clone 2.4G2) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml.
RRID
AB_2892847
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 2.4G2 recognizes the FcγIII and FcγII receptors.
Antigen Distribution
These receptors are present on B cells, monocyte/macrophages, NK cells, neutrophils, mast cells and dendritic cells.
Background
CD16 is expressed in two forms: CD16a and CD16b. CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. CD16b (FcγRIIIB) is a 48 kD GPI-anchored protein whose extracellular domain is over 95% homologous to that of CD16a. CD16 regulates both phagocytosis and antibody-dependent cell-mediated cytotoxicity. It has been reported that CD16 is involved in Natural Killer Cell activation and plays a role in signal transduction. The receptors, CD32 (FcγRIII) and CD16 (FcγRII), are 40-60 kD and bind antibody-antigen immune complexes and mediate adaptive immune responses.

Antigen Details

Protein
Ligand/Receptor
IgG
Function
Low affinity receptors for IgG
PubMed
NCBI Gene Bank ID

References & Citations

1. Titas, J. A. et al. (1982) J. Immunol. 133:556 2. Rodewald, H. et al. (1992) Cell 69:139
IHC
PhenoCycler®
Products are for research use only. Not for use in diagnostic or therapeutic procedures.