Anti-Mouse CD32/CD16 – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Clone 2.4G2 has been validated for use on CODEX® using mouse spleen fresh frozen (FF) tissue.

Sorted pre-B cells

This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.

This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

AB_2892847

Clone 2.4G2 recognizes the FcγIII and FcγII receptors.

These receptors are present on B cells, monocyte/macrophages, NK cells, neutrophils, mast cells and dendritic cells.

CD16 is expressed in two forms: CD16a and CD16b. CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. CD16b (FcγRIIIB) is a 48 kD GPI-anchored protein whose extracellular domain is over 95% homologous to that of CD16a. CD16 regulates both phagocytosis and antibody-dependent cell-mediated cytotoxicity. It has been reported that CD16 is involved in Natural Killer Cell activation and plays a role in signal transduction. The receptors, CD32 (FcγRIII) and CD16 (FcγRII), are 40-60 kD and bind antibody-antigen immune complexes and mediate adaptive immune responses.

IgG

Low affinity receptors for IgG

1. Titas, J. A. et al. (1982) J. Immunol. 133:556 2. Rodewald, H. et al. (1992) Cell 69:139