Anti-Mouse CD32/CD16 [Clone 2.4G2] — Alexa Fluor® 488

This Alexa Fluor® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Alexa Fluor® is a trademark of Life Technologies Corporation.

Liquid

This Alexa Fluor® 488 conjugate is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2-8°C Wet Ice

Blue Laser (488 nm) Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Clone 2.4G2 specifically recognizes a common nonpolymorphic epitope on the extracellular domains of mouse FcγRII (CD32) and FcγRIII (CD16) receptors.

CD16 is expressed in two forms: CD16a and CD16b. CD16a (FcγRIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. CD16b (FcγRIIIB) is a 48 kD GPI-anchored protein whose extracellular domain is over 95% homologous to that of CD16a. CD16 regulates both phagocytosis and antibody-dependent cell-mediated cytotoxicity. It has been reported that CD16 is involved in Natural Killer Cell activation and plays a role in signal transduction. The receptors, CD32 (FcγRIII) and CD16 (FcγRII), are 40-60 kD and bind antibody-antigen immune complexes and mediate adaptive immune responses.

These receptors are present on B cells, monocyte/macrophages, NK cells, neutrophils, mast cells and dendritic cells.

IgG

1. Titas, J. A. et al. (1982) J. Immunol. 133:556 2. Rodewald, H. et al. (1992) Cell 69:139