Anti-Mouse CD8a (Ly 2) – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Anti-Mouse CD8a (Ly 2) – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Product No.: C514

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Clone
53-6.7
Target
CD8a
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Ly2
Isotype
IgG2a
Applications
IHC FF
,
PhenoCycler®

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CODEX® Details

Validation Notes
Clone 53-6.7 has been validated for use on CODEX® using mouse spleen fresh frozen (FF) tissue.
Tissue Screened
Spleen
Tissue Preparation
FF (Fresh Frozen)

Antibody and Reporter Details

Reactivity Species
Mouse
Host Species
Rat
Concentration
0.5 mg/ml
Immunogen
Mouse thymus or spleen
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX® This CD8a (Clone 53-6.7) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml.
RRID
AB_2893571
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 53-6.7 recognizes Lyt 2.
Antigen Distribution
Lyt 2 is present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic cells.
Background
CD8 is made up of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8 is part of the Ig superfamily that expresses primarily as CD8a homodimers. CD8a is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8b. CD8 is an antigen co-receptor on T cells that mediates efficient cell to cell interactions within the immune system. CD8 coupled with the T cell receptor on the T lymphocyte recognizes an antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The CD8 co-receptor also plays a role in T cell signaling by interacting with Lck (lymphocyte-specific protein tyrosine kinase) which leads to the activation of transcription factors that affect the expression of certain genes.

Antigen Details

Protein
Ligand/Receptor
MHC class I molecule
PubMed
NCBI Gene Bank ID

References & Citations

1. Jay Reddy, et al. (2017) J Immunol 2018; 200:523-537 Journal Link
2. Nakayama, K. et al. (1994) Science 263:1131
3. Ledbetter, J. A. et al. (1979) Immunol. Rev. 47:63
4. Ledbetter, J. A. et al. (1980) J. Exp. Med. 152:280
5. Van Ewijk, et al. (1981) J. Immunol. 127:2594
IHC
PhenoCycler®
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.