Anti-Mouse CD8a (Ly 2) (All Alleles) – Biotin

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Pricing & Details

Product No.C216
Clone
53-6.7
Protein
CD8a
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Ly2
Isotype
Rat
IgG2a
Applications
FC
,
IHC FF
,
IHC FFPE
Prod No.
Size
Price
Avail.
Qty
Add to cart
C216-100 µg
100 µg
$60.00
In stock
Max:
Min: 1
Step: 1
C216-500 µg
500 µg
$82.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Data

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Product Concentration
0.5 mg/ml
Formulation
This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this 53-6.7 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Other Applications Reported In Literature ?
IHC (Frozen)
IHC (Paraffin)
Additional Reported Applications For Relevant Conjugates ?
IP
B
Depletion
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 53-6.7 recognizes Lyt 2.
Antigen Distribution
Lyt 2 is present on the surface of most thymocytes and a subpopulation of mature T-lymphocytes which include most T suppressor/cytotoxic cells.
Background
CD8 is made up of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8 is part of the Ig superfamily that expresses primarily as CD8a homodimers. CD8a is a 32-34 kD type I glycoprotein that can also form heterodimers with CD8b. CD8 is an antigen co-receptor on T cells that mediates efficient cell to cell interactions within the immune system. CD8 coupled with the T cell receptor on the T lymphocyte recognizes an antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The CD8 co-receptor also plays a role in T cell signaling by interacting with Lck (lymphocyte-specific protein tyrosine kinase) which leads to the activation of transcription factors that affect the expression of certain genes.

Antigen Details

Protein
Ligand/Receptor
MHC class I molecule
PubMed
NCBI Gene Bank ID

References & Citations

1. Nakayama, K. et al. (1994) Science 263:1131 2. Ledbetter, J. A. et al. (1979) Immunol. Rev. 47:63 3. Ledbetter, J. A. et al. (1980) J. Exp. Med. 152:280 4. Van Ewijk, et al. (1981) J. Immunol. 127:2594
Flow Cytometry
IHC
IHC

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.