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Anti-Mouse CD6 (CD6)
Purified No Carrier Protein
(TP120, T12)
Monoclonal Antibody
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Prod. No.:C1454
Clone:96123
Isotype:Rat IgG2a
Pkg. Size:500 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Rat Anti-Mouse CD6 (Clone 96123) recognizes an epitope on Mouse CD6. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background:
CD6 (Cluster of Differentiation 6) is a cell surface glycoprotein that is involved in T cell activation1 and a member of the scavenger receptor cysteine rich protein superfamily (SRCRSF).2 CD6 can deliver coactivating signals to mature T lymphocytes and regulate its antigen specific and autoreactive responses.3

Host Species
Rat
Immunogen
NS0-Derived Recombinant Mouse CD6 Extracellular Domain
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Goat Anti-Rat IgG (H+L)-Horseradish Peroxidase (Leinco Prod. No.: R380). The detection sensitivity of this indirect ELISA for Mouse CD6 is approximately 1 ng/well.
Western Blotting: To detect Mouse CD6 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rat IgG, Fcγ Fragment Specific-Horseradish Peroxidase (Leinco Prod. No.: R377) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse CD6 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Parnes JR et al. (1995) Eur J Immunol. 25: 2765
2. Bajorath J et al. (1997) Biochemistry 36: 2637
3. Fox DA et al. (1996) Immunology 88: 537
Related Products
Goat Anti-Rat IgG (H+L)-Horseradish Peroxidase (Leinco Prod. No.: R380)
Goat Anti-Rat IgG, Fcγ Fragment Specific-Horseradish Peroxidase (Leinco Prod. No.: R377)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Human CD6 Purified (Leinco Prod. No.: C421)
Anti-Human CD6 (Antigen Aff. Pur.) (Leinco Prod. No.: C1471)
Anti-Human CD6-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: C1516)
Recombinant Human CD6 (Leinco Prod. No.: C1348)
Recombinant Mouse CD6 (Leinco Prod. No.: C1353)
Anti-Human CD6 (Leinco Prod. No.: C1446)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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