Anti-Mouse CD6
Anti-Mouse CD6
Product No.: C1454
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Clone 96123 Target CD6 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names TP120, T12 Isotype IgG2a Applications CyTOF® , FC , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen NS0-Derived Recombinant Mouse CD6 Extracellular Domain Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828517 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse CD6 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CD6 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Flow Cytometry: Suitable for use at concentration of 2.5 µg/106 cells. Additional Applications Reported In Literature ? CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rat Anti-Mouse CD6 (Clone 96123) recognizes an epitope on Mouse CD6. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background CD6 (Cluster of Differentiation 6) is a cell surface glycoprotein that is involved in T cell activation1 and a member of the scavenger receptor cysteine rich protein superfamily (SRCRSF).2 CD6 can deliver coactivating signals to mature T lymphocytes and regulate its antigen specific and autoreactive responses.3 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Parnes, JR. et al. (1995) Eur J Immunol. 25: 2765 2. Bajorath, J. et al. (1997) Biochemistry 36: 2637 3. Fox, DA. et al. (1996) Immunology 88: 537 Technical ProtocolsCyTOF®   |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
