NS0 - Derived Recombinant Human CD6 Extracellular Domain
<0.1 EU/µg as determined by the LAL method
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human CD6 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for rhCD6 is approximately 0.5 ng/lane and 2 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Flow Cytometry: Suitable for use at concentration of 2.5 µg/106 cells
Other Applications Reported In Literature ?
Blocking: The adhesion of HuT 78 human cutaneous T cell lymphoma cells (5 x 104 cells/well) to immobilized Recombinant Human CD6 Fc Chimera was maximally inhibited (80-100%) by 50 µg/mL of the antibody.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Goat Anti-Human CD6 recognizes Human CD6. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
CD6 (Cluster of Differentiation 6) is a cell surface glycoprotein that is involved in T cell activation1 and a member of the scavenger receptor cysteine rich protein superfamily (SRCRSF).2 CD6 can deliver coactivating signals to mature T lymphocytes and regulate its antigen specific and autoreactive responses.3
NCBI Gene Bank ID
References & Citations
1. Parnes, JR. et al. (1995) Eur J Immunol. 25: 2765
2. Bajorath, J. et al. (1997) Biochemistry 36: 2637
3. Fox, DA. et al. (1996) Immunology 88: 537
Products are for research use only. Not for use in diagnostic or therapeutic procedures.