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Goat Anti-Mouse VCAM-1
Biotin
(Vascular Cell Adhesion Molecule 1, CD106, INCAM-100, VLA4)
Polyclonal Antibody
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Prod. No.:V161
Host:Goat
Pkg. Size:50 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Goat Anti-Mouse Vascular Cell Adhesion Molecule 1 (VCAM-1) recognizes Mouse VCAM-1. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background:
Vascular cell adhesion molecule 1, also known as VCAM1 is an immunoglobulin-like adhesion molecule expressed on activated endothelial cells.1 This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. VCAM-1 promotes the adhesion of lymphocytes, monocytes, eosinophils, and basophils.

Known Species Cross Reactivity: Ms+
Immunogen
Purified Recombinant Mouse VCAM-1 (>98%)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Reconstitution
We suggest aseptically reconstituting this biotinylated, lyophilized, antigen affinity purified polyclonal antibody with 1 ml of sterile 20 mM Trizma base, 0.15 M sodium chloride (1X Tris Buffered Saline) pH 7.2-7.3, containing 0.1% w/v bovine serum albumin (BSA). This will result in a final biotinylated antibody concentration of 50 µg/ml.
Storage and Stability
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted bioin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Reported Applications
WB
Western Blotting: To detect Mouse VCAM-1 this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for Mouse VCAM-1 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is Recombinant Mouse VCAM-1 (Leinco Prod. No.: V125).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Yuqing Huo et al. (2001) J Clin Invest 107: 1209
Related Products
UltraAvidin™- Horseradish Peroxidase (HRPO) (Leinco Prod. No.: A106)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Recombinant Mouse VCAM-1 (Leinco Prod. No.: V125)
Anti-Mouse VCAM-1 (Leinco Prod. No.: V145)
Anti-Mouse VCAM-1 (Antigen Aff. Pur.) (Leinco Prod. No.: V150)
Anti-Human VCAM-1 (Antigen Aff. Pur.) (Leinco Prod. No.: V154)
Anti-Human VCAM-1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: V164)
Recombinant Human VCAM-1 (Leinco Prod. No.: V169)
Recombinant Human VCAM-1 (Leinco Prod. No.: V129)
Recombinant Human VCAM-1 (Leinco Prod. No.: V168)
Anti-Mouse VCAM-1 (Leinco Prod. No.: V144)
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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