Recombinant Human VCAM-1

Recombinant Human VCAM-1

Product No.: V129

[product_table name="All Top" skus="V129"]

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Prod. No.V129
Expression Host
NS0 Cells

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Background

Vascular cell adhesion molecule 1, also known as VCAM1 is an immunoglobulin-like adhesion molecule expressed on activated endothelial cells.1 This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. VCAM-1 promotes the adhesion of lymphocytes, monocytes, eosinophils, and basophils.

Protein Details

Purity
>90% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human VCAM-1 was determined by its ability to support adhesion of U937 human histiocytic lymphoma cells.
Protein Accession No.
Amino Acid Sequence
mfkiett pesrylaqig dsvsltcstt gcespffswr tqidsplngk vtnegttstl tmnpvsfgne hsylctatce srklekgiqv eiysfpkdpe ihlsgpleag kpitvkcsva dvypfdrlei dllkgdhlmk sqefledadr ksletkslev tftpviedig kvlvcraklh idemdsvptv rqavkelqvy ispkntvisv npstklqegg svtmtcsseg lpapeifwsk kldngnlqhl sgnatltlia mrmedsgiyv cegvnligkn rkevelivqe kpftveispg priaaqigds vmltcsvmgc espsfswrtq idsplsgkvr segtnstltl spvsfenehs ylctvtcghk klekgiqvel ysfprdpeie msgglvngss vtvsckvpsv ypldrleiel lkgetileni efledtdmks lenkslemtf iptiedtgka lvcqaklhid dmefepkqrq stqtlyvnva prdttvlvsp ssileegssv nmtclsqgfp apkilwsrql pngelqplse natltlistk medsgvylce ginqagrsrk eveliiqvtp kdikltafps esvkegdtvi isctcgnvpe twiilkkkae tgdtvlksid gaytirkaql kdagvyeces knkvgsqlrs ltldvqgren nkdyfspe
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human VCAM-1 is Mr 74 kDa. However, the actual molecular weight as observed by migration onSDS-PAGE is Mr 90-95 kDa.
Predicted Molecular Mass
74
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Huo, Y. et al. (2001) J Clin Invest 107: 1209
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.