Recombinant Human VCAM-1

Vascular cell adhesion molecule 1, also known as VCAM1 is an immunoglobulin-like adhesion molecule expressed on activated endothelial cells.¹ This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. VCAM-1 promotes the adhesion of lymphocytes, monocytes, eosinophils, and basophils.

The biological activity of Human VCAM-1 was determined by its ability to support adhesion of U937 human histiocytic lymphoma cells.

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Lyophilized

The predicted molecular weight of Recombinant Human VCAM-1 is Mr 74 kDa. However, the actual molecular weight as observed by migration onSDS-PAGE is Mr 90-95 kDa.

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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1. Huo, Y. et al. (2001) J Clin Invest 107: 1209