Recombinant Human VCAM-1

Vascular cell adhesion molecule 1, also known as VCAM1 is an immunoglobulin-like adhesion molecule expressed on activated endothelial cells. A member of the immunoglobulin superfamily this type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. VCAM-1 promotes the adhesion of lymphocytes, monocytes, eosinophils, and basophils.

The biological activity of Human VCAM-1 was determined by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. Add 5 X 104 cells/well to human VCAM-1 coated plates (10 µg/ml with 100 µl/well), approximately 85-95% will adhere after 1 hour incubation at room temperature.

P19320-1

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Lyophilized

The predicted molecular weight of Recombinant Human CD106, INCAM-100, MGC108734, MGC99561, VCAM, VCAM1 is Mr 74.1 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 93-95 kDa, reducing conditions kDa.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Flow Cytometry: PN:A106
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCR5. A suggested method would be to stain cells expressing CXCR5 with approximately 10 µl per test. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 10⁵ continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes. Labeling of the cells with the biotin conjugate should be followed by PN:A104, resuspended in 200-400 µl of 1X PBS.