Vascular cell adhesion molecule 1, also known as VCAM1 is an immunoglobulin-like adhesion molecule expressed on activated endothelial cells. A member of the immunoglobulin superfamily this type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. VCAM-1 promotes the adhesion of lymphocytes, monocytes, eosinophils, and basophils.
>90% by SDS Page and analyzed by silver stain.
<0.1 EU/µg as determined by the LAL method
The biological activity of Human VCAM-1 was determined by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. Add 5 X 104 cells/well to human VCAM-1 coated plates (10 µg/ml with 100 µl/well), approximately 85-95% will adhere after 1 hour incubation at room temperature.
Protein Accession No.
Amino Acid Sequence
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State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human CD106, INCAM-100, MGC108734, MGC99561, VCAM, VCAM1 is Mr 74.1 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 93-95 kDa, reducing conditions kDa.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
NCBI Gene Bank
Applications and Recommended Usage ?
(Quality Tested by Leinco)
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Flow Cytometry: PN:A106
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CXCR5. A suggested method would be to stain cells expressing CXCR5 with approximately 10 µl per test. A typical test sample constitutes approximately 50 µl of packed whole blood or 1 x 105 continuous passage or activated cell cultures that have been centrifuged at 500 X g for five minutes. Labeling of the cells with the biotin conjugate should be followed by PN:A104, resuspended in 200-400 µl of 1X PBS.
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.