Anti-Mouse VCAM-1 (Clone 112734)

Anti-Mouse VCAM-1 (Clone 112734)

Product No.: V144

[product_table name="All Top" skus="V144"]

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Clone
112734
Target
VCAM-1
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Vascular Cell Adhesion Molecule 1, CD106, INCAM-100, VLA4
Isotype
IgG2a
Applications
CyTOF®
,
ELISA Cap
,
FC
,
WB

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
NS0-Derived Recombinant Mouse VCAM-1
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2.0 µg/ml. A suitable detection antibody is PN:V161 at a concentration of approximately 200 µg/ml. A suggested standard for this assay is PN:V125. No cross reactivity was observed with human VCAM-1 or other forms.
Western Blotting: To detect Mouse VCAM-1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for rmVCAM-1 is approximately 1 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Simple Western: Suitable for use at concentration of 100 µg/mL.
Flow Cytometry: Suitable for use at concentration of 0.25 µg/106 cells.
Additional Applications Reported In Literature ?
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse Vascular Cell Adhesion Molecule 1 (VCAM-1) (Clone 112734) recognizes an epitope on Mouse VCAM-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Vascular cell adhesion molecule 1, also known as VCAM1 is an immunoglobulin-like adhesion molecule expressed on activated endothelial cells.1 This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. VCAM-1 promotes the adhesion of lymphocytes, monocytes, eosinophils, and basophils.

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Huo, Y. et al. (2001) J Clin Invest 107: 1209
CyTOF®
Elisa Sandwich Protocol
Flow Cytometry
General Western Blot Protocol

Certificate of Analysis

Formats Available

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Prod No.
Description
V144
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.