Recombinant Human VCAM-1

Vascular cell adhesion molecule 1, also known as VCAM1 is an immunoglobulin-like adhesion molecule expressed on activated endothelial cells. A member of the immunoglobulin superfamily this type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. VCAM-1 promotes the adhesion of lymphocytes, monocytes, eosinophils, and basophils.

The biological activity of Human VCAM-1 was determined by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. Add 5 X 10⁴ cells/well to human VCAM-1 coated plates (10 µg/ml with 100 µl/well), approximately 90-100% will adhere after 2 hour incubation at room temperature. The expected ED₅₀= , corresponding to a specific activity of 

Fc Fusion Protein

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Met

Lyophilized

The predicted molecular weight of Recombinant Human VCAM-1 is Mr 101.6 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 130-140 kDa.

101.6

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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