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Anti-Human VCAM-1
Purified No Carrier Protein
(Vascular Cell Adhesion Molecule 1, CD106, INCAM-100, VLA4)
Monoclonal Antibody
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Prod. No.:V145
Clone:112702
Isotype:Rat IgG1
Pkg. Size:500 µg, 1 mg
Storage:-20°C to -70°C Detailed storage instructions below.
Description
Specificity:
Rat Anti-Human Vascular Cell Adhesion Molecule 1 (VCAM-1) (Clone 112702) recognizes an epitope on Human VCAM-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background:
Vascular cell adhesion molecule 1, also known as VCAM1 is an immunoglobulin-like adhesion molecule expressed on activated endothelial cells.1 This type I membrane protein mediates leukocyte-endothelial cell adhesion and signal transduction, and may play a role in the development of artherosclerosis and rheumatoid arthritis. VCAM-1 promotes the adhesion of lymphocytes, monocytes, eosinophils, and basophils.

Host Species
Rat
Immunogen
NS0-Derived Recombinant Mouse VCAM-1 Extracellular Domain
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Reconstitution
This lyophilized antibody may be aseptically reconstituted with sterile PBS to a final volume of 1.0 ml. This will result in a final antibody concentration of 500 µg/ml.
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Reported Applications
Indirect ELISA, WB
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as Goat Anti-Rat IgG (H+L)-Horseradish Peroxidase (Leinco Prod. No.: R380). The detection sensitivity of this indirect ELISA for Human VCAM-1 is approximately 1 ng/well.
Western Blotting: To detect Human VCAM-1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as Goat Anti-Rat IgG, Fcγ Fragment Specific-Horseradish Peroxidase (Leinco Prod. No.: R377) and a chromogenic substrate such as TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343). The detection limit for rhVCAM-1 is approximately 0.5 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Yuqing Huo et al. (2001) J Clin Invest 107: 1209
Related Products
Goat Anti-Rat IgG (H+L)-Horseradish Peroxidase (Leinco Prod. No.: R380)
Goat Anti-Rat IgG, Fcγ Fragment Specific-Horseradish Peroxidase (Leinco Prod. No.: R377)
TMB HRPO Membrane/IHC Substrate UltraSensitive™ One Component "Ready Use" (Leinco Prod. No.: T343)
Anti-Mouse VCAM-1 (Antigen Aff. Pur.) (Leinco Prod. No.: V150)
Anti-Human VCAM-1 (Antigen Aff. Pur.) (Leinco Prod. No.: V154)
Anti-Mouse VCAM-1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: V161)
Anti-Human VCAM-1-Biotin (Antigen Aff. Pur.) (Leinco Prod. No.: V164)
Recombinant Human VCAM-1 (Leinco Prod. No.: V169)
Recombinant Human VCAM-1 (Leinco Prod. No.: V129)
Recombinant Mouse VCAM-1 (Leinco Prod. No.: V125)
Recombinant Human VCAM-1 (Leinco Prod. No.: V168)
Anti-Mouse VCAM-1 (Leinco Prod. No.: V144)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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