Mouse Anti-Human CD3 (Clone UCHT-1) recognizes an epitope on Human CD3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
The human CD3 antigen is expressed on approximately 60-80% of human peripheral blood lymphocytes, 20-40% of splenic lymphocytes, the majority of T-CLL and approximately 70% of T-ALL cells.
Anti-CD3 may be used for enumerating immunocompetent T-lymphocytes in peripheral blood. Clone UCHT-1 is also useful in histology for localization of T-lymphocytes in tissue and may be used to enrich T-cells by cell sorting. This T-cell marker has also been used to determine T-versus B-cell lymphomas and leukemias.
DyLight® 650 conjugated antibodies absorb light maximally around 652 nm (± 4 nm) and fluoresce maximally around 672 nm. They are brighter than Cy5 and Alexa 647 conjugates plus, they typically give less background than Alexa 647 conjugates. DyLight® 650 antibody conjugates are the best choice for far red-emitting conjugates and for multiple-labeling detection with a confocal microscope.
DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
Known Species Cross Reactivity: Hu+
Purified Recombinant Human CD3 (>98%)
This DyLight® 650 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This DyLight® 650 conjugate is stable when stored at 2-8°C. Do not freeze.
Flow Cytometry: This antibody can be used at a concentration of ≤ 0.25 µg per 100 µl of whole blood (or per 1.0 x 106 cells in a 100 µl total staining volume).
Each investigator should determine their own optimal working dilution for specific applications.
Country of Origin
1. Leukocyte Typing VI (1996) Oxford University Press
2. van Dongen, J. J. M. et al. (1988) Blood71:603
3. Meuer, S. C. et al. (1983) Nature303:808
4. Beverly, P.C.L. et al. (1981) Eur. J. Immunol.11:329
5. Leukocyte Typing III: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2