Anti-Human CD3 (Clone UCHT-1) – Purified in vivo GOLD™ Functional Grade

Pricing & Details

Product No.C364
Clone
UCHT-1
Protein
CD3
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
T3, CD3ε
Isotype
Mouse
IgG1 κ
Applications
Act
,
CODEX®
,
CyTOF®
,
FC
,
IF Staining
,
IHC FF
,
IP
,
WB
Workshops
III 471
Prod No.
Size
Price
Avail.
Qty
Add to cart
C364-1.0 mg
1.0 mg
$105.00
$81.90
In stock
Max:
Min: 1
Step: 1
C364-5.0 mg
5.0 mg
$355.00
$276.90
In stock
Max:
Min: 1
Step: 1
C364-25 mg
25 mg
$965.00
$752.70
In stock
Max:
Min: 1
Step: 1
C364-50 mg
50 mg
$1,595.00
$1,244.10
In stock
Max:
Min: 1
Step: 1
C364-100 mg
100 mg
$2,300.00
$1,794.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
This antibody was created by Professor Peter Beverley, a pioneer in creating hybridomas from mice immunized against human lymphocytes, with UCHT1 being one of the first successful fusions.
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this UCHT-1 antibody for staining cells in flow cytometry is ≤ .25 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
WB
Other Applications Reported In Literature ?
CODEX®
CyTOF®
IP
Activation
IHC (Frozen) The suggested concentration for this UCHT-1 antibody in IHC staining on frozen tissue is 5.0 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone UCHT-1 binds to an acidic region of CD3-epsilon, occluding this region from direct interaction with the T cell receptor. This antibody is considered a pan T-cell marker. Furthermore, this antibody can be used for the detection of T cell populations in peripheral blood, lymph nodes and the categorisation of T versus B cell lymphomas and leukaemia’s. It reacts with the majority of peripheral blood T lymphocytes, a significant proportion of thymocytes, the majority of T cell chronic lymphocytic leukemia cells and approximately 70% of acute lymphoblastic leukaemia’s of T cell origin.
Antigen Distribution
CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells.
Background
CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Antigen Details

Protein
Ligand/Receptor
Peptide antigen bound to MHC
Function
Antigen recognition, signal transduction, T cell activation
PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. Hitsuda, Y. et al. (1999) Yonago Acta medica 42:1–10 Article Link
2. van Dongen, J. et al. (1988) Blood 71:603
3. Meuer, SC. et al. (1983) Nature 303:808
4. Beverly, P. et al. (1981) Eur. J. Immunol. 11:329
5. Leukocyte Typing III: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2
Flow Cytometry
IHC
Immunoprecipitation Protocol
General Western Blot Protocol