Anti-Human CD3 – Purified In vivo GOLD™ Functional Grade
Pricing & Details
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Clone UCHT-1 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names T3, CD3ε Isotype Mouse IgG1 κ Applications CODEX® , CyTOF® , FC , IF Staining , IHC FF Workshops III 471  |
Antibody DetailsProduct DetailsMatched Isotype Control Reactivity Species Human Host Species Mouse Immunogen Unknown Product Concentration ≥ 2.0 mg/ml Endotoxin Level <0.1 EU/µg as determined by the LAL method Purity >95% by SDS-PAGE and HPLC Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium, calcium or preservatives added. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Applications and Recommended Usage Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD3. A suggested method would be to stain cells expressing CD3 with 2.0 µg per million cells in a total staining volume of 100 µl followed by PN:M1189. Applications and Recommended Usage ? (Quality Tested by Leinco) FC The suggested concentration for this UCHT-1 antibody for staining cells in flow cytometry is ≤ .25 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. Other Applications Reported In Literature ? CODEX® CyTOF® IHC (Frozen) The suggested concentration for this UCHT-1 antibody in IHC staining on frozen tissue is 5.0 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Clone UCHT-1 recognizes an epitope on human CD3ε. Antigen Distribution CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells. Background CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition. Antigen DetailsProtein CD3 Ligand/Receptor Peptide antigen bound to MHC Function Antigen recognition, signal transduction, T cell activation PubMed CD3+AND+UCHT-1 NCBI Gene Bank ID UniProt.org References & Citations1. Hitsuda, Y. et al. (1999) Yonago Acta medica 42:1–10 Article Link 2. van Dongen, J. et al. (1988) Blood 71:603 3. Meuer, SC. et al. (1983) Nature 303:808 4. Beverly, P. et al. (1981) Eur. J. Immunol. 11:329 5. Leukocyte Typing III: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2 Technical Protocols  |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.