Anti-Human CD3 – Purified In vivo GOLD™ Functional Grade

Unknown

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium, calcium or preservatives added.

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

FC The suggested concentration for this UCHT-1 antibody for staining cells in flow cytometry is ≤ .25 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.

CODEX®
CyTOF®
IHC (Frozen) The suggested concentration for this UCHT-1 antibody in IHC staining on frozen tissue is 5.0 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application.

Clone UCHT-1 recognizes an epitope on human CD3ε.

CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells.

CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Peptide antigen bound to MHC

Antigen recognition, signal transduction, T cell activation

CD3+AND+UCHT-1

1. Hitsuda, Y. et al. (1999) Yonago Acta medica 42:1–10 Article Link
2. van Dongen, J. et al. (1988) Blood 71:603
3. Meuer, SC. et al. (1983) Nature 303:808
4. Beverly, P. et al. (1981) Eur. J. Immunol. 11:329
5. Leukocyte Typing III: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2