Anti-Human CD3 (Clone UCHT-1) – Purified in vivo GOLD™ Functional Grade
Pricing & Details
Antibody DetailsProduct DetailsReactivity Species Human Host Species Mouse Immunogen Unknown Product Concentration ≥ 5.0 mg/ml Endotoxin Level ≤ 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829677 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this UCHT-1 antibody for staining cells in flow cytometry is ≤ .25 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. WB Other Applications Reported In Literature ? CODEX® CyTOF® IP Activation IHC (Frozen) The suggested concentration for this UCHT-1 antibody in IHC staining on frozen tissue is 5.0 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Clone UCHT-1 recognizes an epitope on human CD3ε. Antigen Distribution CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells. Background CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition. Antigen DetailsProtein Ligand/Receptor Peptide antigen bound to MHC Function Antigen recognition, signal transduction, T cell activation PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology References & Citations1. Hitsuda, Y. et al. (1999) Yonago Acta medica 42:1–10 Article Link 2. van Dongen, J. et al. (1988) Blood 71:603 3. Meuer, SC. et al. (1983) Nature 303:808 4. Beverly, P. et al. (1981) Eur. J. Immunol. 11:329 5. Leukocyte Typing III: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2 Technical Protocols    |