Anti-Human CD3 – Purified In vivo GOLD™ Functional Grade

Pricing & Details

  
Clone
UCHT-1
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
T3, CD3ε
Isotype
Mouse
IgG1 κ
Applications
CODEX®
,
CyTOF®
,
FC
,
IF Staining
,
IHC FF
Workshops
III 471
Prod No.
Size
Price
Avail.
Qty
Add to cart
C364-1.0 mg
1.0 mg
$95.00
In stock
C364-5.0 mg
5.0 mg
$298.00
In stock
C364-25 mg
25 mg
$775.00
In stock
C364-50 mg
50 mg
$1,275.00
In stock
C364-100 mg
100 mg
$1,998.00
In stock
  

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Unknown
Product Concentration
≥ 2.0 mg/ml
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Applications and Recommended Usage
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD3. A suggested method would be to stain cells expressing CD3 with 2.0 µg per million cells in a total staining volume of 100 µl followed by PN:M1189.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
FC The suggested concentration for this UCHT-1 antibody for staining cells in flow cytometry is ≤ .25 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Other Applications Reported In Literature ?
CODEX®
CyTOF®
IHC (Frozen) The suggested concentration for this UCHT-1 antibody in IHC staining on frozen tissue is 5.0 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone UCHT-1 recognizes an epitope on human CD3ε.
Antigen Distribution
CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells.
Background
CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Antigen Details

Protein
CD3
Ligand/Receptor
Peptide antigen bound to MHC
Function
Antigen recognition, signal transduction, T cell activation
PubMed
CD3+AND+UCHT-1
NCBI Gene Bank ID

References & Citations

1. Hitsuda, Y. et al. (1999) Yonago Acta medica 42:1–10 Article Link
2. van Dongen, J. et al. (1988) Blood 71:603
3. Meuer, SC. et al. (1983) Nature 303:808
4. Beverly, P. et al. (1981) Eur. J. Immunol. 11:329
5. Leukocyte Typing III: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2
Flow Cytometry
IHC
  

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.