Anti-Human CD3 – Purified

Pricing & Details

Product No.C105
Clone
UCHT-1
Protein
CD3
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
Mouse
IgG1
Applications
Act
,
FC
,
IF Staining
,
IHC FF
,
IP
,
PhenoCycler®
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C105-25µg
25 µg
$22.00
In stock
Max:
Min: 1
Step: 1
C105-50 µg
50 µg
$55.00
In stock
Max:
Min: 1
Step: 1
C105-100 µg
100 µg
$93.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this UCHT-1 antibody for staining cells in flow cytometry is ≤ .25 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
WB
Other Applications Reported In Literature ?
CODEX®
Activation
IHC (Frozen) The suggested concentration for this UCHT-1 antibody in IHC staining on frozen tissue is 5.0 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone UCHT-1 recognizes an epitope on human CD3ε.
Antigen Distribution
CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells.
Background
CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Antigen Details

Protein
Ligand/Receptor
Peptide antigen bound to MHC
PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. Aliño, SF. et al. (2002) J Control Release. 83(1):133-46. PubMed
2. Yamada, T. et al. (2003) J Immunol 170:4441-9Article Link
3. Hitsuda, Y. et al. (1999) Yonago Acta medica 42:1–10 Article Link
4. van Dongen, J. et al. (1988) Blood 71:603
5. Meuer, SC. et al. (1983) Nature 303:808
6. Beverly, P. et al. (1981) Eur. J. Immunol. 11:329
7. Leukocyte Typing III: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2
Flow Cytometry
IHC
Immunoprecipitation Protocol
PhenoCycler®
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.