Anti-Human CD3 – Purified

Pricing & Details

  
Clone
UCHT-1
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
Mouse
IgG1
Applications
CyTOF®
,
FC
,
IF Staining
,
IHC FF
,
IP
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C105-50 µg
50 µg
$50.00
In stock
C105-100 µg
100 µg
$85.00
In stock
C105-200 µg
200 µg
$150.00
In stock
  

Antibody Details

Reactivity Species
Human
Host Species
Mouse
Product Concentration
0.1 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This antibody is stable when stored at 2-8°C. Do not freeze.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
FC The suggested concentration for this UCHT-1 antibody for staining cells in flow cytometry is ≤ .25 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
Flow Cytometry: This antibody has been quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. Each investigator should determine their own optimal working dilution for specific applications.
Other Applications Reported In Literature ?
CODEX®
CyTOF®
IHC (Frozen) The suggested concentration for this UCHT-1 antibody in IHC staining on frozen tissue is 5.0 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application.
Each investigator should determine their own optimal working dilution for specific applications.

Description

Specificity
Clone UCHT-1 recognizes an epitope on human CD3ε.
Antigen Distribution
CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells.
Background
CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Antigen Details

Protein
CD3
Ligand/Receptor
Peptide antigen bound to MHC
PubMed
CD3+AND+UCHT-1
NCBI Gene Bank ID

References & Citations

1. Aliño, SF. et al. (2002) J Control Release. 83(1):133-46. PubMed
2. Yamada, T. et al. (2003) J Immunol 170:4441-9Article Link
3. Hitsuda, Y. et al. (1999) Yonago Acta medica 42:1–10 Article Link
4. van Dongen, J. et al. (1988) Blood 71:603
5. Meuer, SC. et al. (1983) Nature 303:808
6. Beverly, P. et al. (1981) Eur. J. Immunol. 11:329
7. Leukocyte Typing III: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2
Flow Cytometry
IHC
Immunoprecipitation Protocol
General Western Blot Protocol
  

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.