Anti-Human CD3 – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Anti-Human CD3 – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Product No.: C504

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Clone
UCHT-1
Target
CD3
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
IgG1
Applications
IHC FF
,
PhenoCycler®

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Product Size
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CODEX® Details

Validation Notes
Clone UCHT-1 has been validated for use on CODEX® using human tonsil fresh frozen (FF) tissue.
Tissue Screened
Tonsil
Tissue Preparation
FF (Fresh Frozen)

Antibody and Reporter Details

Reactivity Species
Human
Host Species
Mouse
Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX® This CD3 (Clone UCHT-1) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml.
RRID
AB_2893565
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone UCHT-1 recognizes an epitope on human CD3ε.
Antigen Distribution
CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells.
Background
CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.

Antigen Details

Protein
Ligand/Receptor
Peptide antigen bound to MHC
PubMed
NCBI Gene Bank ID

References & Citations

1. Aliño, SF. et al. (2002) J Control Release. 83(1):133-46. PubMed
2. Yamada, T. et al. (2003) J Immunol 170:4441-9Article Link
3. Hitsuda, Y. et al. (1999) Yonago Acta medica 42:1–10 Article Link
4. van Dongen, J. et al. (1988) Blood 71:603
5. Meuer, SC. et al. (1983) Nature 303:808
6. Beverly, P. et al. (1981) Eur. J. Immunol. 11:329
7. Leukocyte Typing III: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2
IHC
PhenoCycler®

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.