Anti-Human CD3 – PerCP/Cy5.5

Anti-Human CD3 – PerCP/Cy5.5

Product No.: C2134

[product_table name="All Top" skus="C2134"]

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Clone
UCHT-1
Target
CD3
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
IgG1
Applications
FC

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Product Concentration
0.1 mg/ml
Formulation
This PerCp/Cy5.5 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This PerCp/Cy5.5 conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this UCHT-1 antibody for staining cells in flow cytometry is ≤ .25 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Clone UCHT-1 recognizes an epitope on human CD3ε.
Background
CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.
Antigen Distribution
CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells.
Ligand/Receptor
Peptide antigen bound to MHC
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. van Dongen, J. et al. (1988) Blood 71:603
2. Meuer, SC. et al. (1983) Nature 303:808
3. Beverly, P. et al. (1981) Eur. J. Immunol. 11:329
4. Leukocyte Typing III: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2
Flow Cytometry

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.