Anti-Human CD119 (IFN-γ Rα Chain) – Purified in vivo GOLD™ Functional Grade
Anti-Human CD119 (IFN-γ Rα Chain) – Purified in vivo GOLD™ Functional Grade
Product No.: I-443
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Clone GIR-208 Target IFNγ Rα Formats AvailableView All Product Type Monoclonal Antibody Alternate Names IFNGR1, IFNg Ra Isotype IgG1 Applications FA , FC , in vivo |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Human IFN-γRα, Purified from human placenta Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737544 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for clone GIR-208 purified antibody for staining cells in flow cytometry is ≤ 0.5 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? FA The In vivo GOLD™ Purified antibody is recommended for functional assays. Clone GIR-208 can be used as an isotype with Clone MAR1-5A3 (Anti-Mouse IFNAR1) Additional Reported Applications For Relevant Conjugates ? WB IP IHC B Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Clone GIR-208 recognizes an epitope on human IFNγ Rα. Antigen Distribution The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes. Background IFNγ Rα, or IFNγ R1, is a 90-100 kD type I transmembrane protein that is structurally related to IL-10 receptor. IFNγ Receptor consists of α and ß chains and requires association of JAK1, JAK2 and Stat1 for IFN-γ signal transduction which induces tyrosine phosphorylation of IFN-γ Rα leading to the formation of a docking site on the activated receptor for Stat1, which specifically activates IFN-γ induced gene transcription. Antigen DetailsResearch Area Immunology References & Citations1. Schreiber, RD. et al. (2017) Cancer Immunol Res. 5(2):106-117. PubMed 2. Schreiber, RD. et al. (2015) PLoS One.10(5):e0128636. PubMed Technical ProtocolsFA   |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
