Anti-Human CD119 (IFN-γ Rα Chain) – Purified In vivo GOLD™ Functional Grade

Pricing & Details

Product No.I-443
Clone
GIR-208
Protein
IFNγ Rα
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
IFNGR1, IFNg Ra
Isotype
Mouse
IgG1
Applications
FA
,
FC
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-443-1.0 mg
1.0 mg
$95.00
In stock
Max:
Min: 1
Step: 1
I-443-5.0 mg
5.0 mg
$298.00
In stock
Max:
Min: 1
Step: 1
I-443-25 mg
25 mg
$800.00
In stock
Max:
Min: 1
Step: 1
I-443-50 mg
50 mg
$1,325.00
In stock
Max:
Min: 1
Step: 1
I-443-100 mg
100 mg
$1,998.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Human IFN-γRα, Purified from human placenta
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for clone GIR-208 purified antibody for staining cells in flow cytometry is ≤ 0.5 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Other Applications Reported In Literature ?
FA The In vivo GOLD™ Purified antibody is recommended for functional assays. Clone GIR-208 can be used as an isotype with Clone MAR1-5A3 (Anti-Mouse IFNAR1)
Additional Reported Applications For Relevant Conjugates ?
WB
IP
IHC
B
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone GIR-208 recognizes an epitope on human IFNγ Rα.
Antigen Distribution
The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes.
Background
IFNγ Rα, or IFNγ R1, is a 90-100 kD type I transmembrane protein that is structurally related to IL-10 receptor. IFNγ Receptor consists of α and ß chains and requires association of JAK1, JAK2 and Stat1 for IFN-γ signal transduction which induces tyrosine phosphorylation of IFN-γ Rα leading to the formation of a docking site on the activated receptor for Stat1, which specifically activates IFN-γ induced gene transcription.

Antigen Details

Protein
PubMed
Research Area
Immunology

References & Citations

1. Schreiber, RD. et al. (2017) Cancer Immunol Res. 5(2):106-117. PubMed
2. Schreiber, RD. et al. (2015) PLoS One.10(5):e0128636. PubMed
Flow Cytometry

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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