This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
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Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for clone GIR-208 purified antibody for staining cells in flow cytometry is ≤ 0.5 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Other Applications Reported In Literature ?
FA The Purified antibody is recommended for functional assays. Clone GIR-208 can be used as an isotype with Clone MAR1-5A3 (Anti-Mouse IFNAR1)
Additional Reported Applications For Relevant Conjugates ?
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Clone GIR-208 recognizes an epitope on human IFNγ Rα.
The IFN-γ receptor is expressed at moderate levels on virtually every cell with the exception of erythrocytes.
IFNγ Rα, or IFNγ R1, is a 90-100 kD type I transmembrane protein that is structurally related to IL-10 receptor. IFNγ Receptor consists of α and ß chains and requires association of JAK1, JAK2 and Stat1 for IFN-γ signal transduction which induces tyrosine phosphorylation of IFN-γ Rα leading to the formation of a docking site on the activated receptor for Stat1, which specifically activates IFN-γ induced gene transcription.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.