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Anti-Human CD71 (CD71)
Fluorescein (FITC)
(Transferrin Receptor)
Monoclonal Antibody
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Prod. No.:C165
Clone:T56/14
Isotype:Mouse IgG1
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human CD71 (Clone T56/14) recognizes Human CD71 transferrin receptor (Mr 190 kDa). This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:
The transferrin receptor is a cell surface glycoprotein related to cell proliferation. Up-regulation of the CD71 antigen is associated with actively dividing cells.1 Transferrin receptors are also present on reticulocytes, but are lost during maturation into erythrocytes.2

Background:
Anti-transferrin receptor may be used to identify activated lymphocytes. The CD71 antibody may be used in studies of erythropoiesis.

Reporter Molecule:
Fluorescein (FITC) is a small fluorescent organic molecule with a molecular weight of 473 daltons with an absorbance maximum of ~492 nm and an emission maximum of ~518 nm.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Formulation
This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Flow Cytometry: This antibody can be used at a concentration of ≤ 1.0 µg per 100 µl of whole blood (or per 1.0 x 106 cells in a 100 µl total staining volume).
Flow Cytometry: This antibody can be used at a concentration of ≤ 1.0 µg per 100 µl of whole blood (or per 1.0 x 106 cells in a 100 µl total staining volume).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Trowbridge, S. I. et al. (1981) Proc. Nat'l. Acad. Sci. 78:3039
2. Iacopetta, B. J. et al. (1983) J. Histochem. Cytochem. 31:336
Related Products
Anti-Human CD71 (Transferrin Receptor)-Biotin (Leinco Prod. No.: C164)
Anti-Human CD71 (Transferrin Receptor)-R-phycoerythrin (R-PE) (Leinco Prod. No.: C166)
Anti-Human CD71 (Transferrin Receptor)-Allophycocyanin (APC) (Leinco Prod. No.: C328)
Anti-Mouse CD71 (Transferrin Receptor) Purified (Leinco Prod. No.: C275)
Anti-Mouse CD71 (Transferrin Receptor)-Fluorescein (Leinco Prod. No.: C277)
Anti-Mouse CD71 (Transferrin Receptor) Purified Low Endotoxin Functional Formulation (Leinco Prod. No.: C353)
Anti-Mouse CD71 (Transferrin Receptor)-Allophycocyanin (Leinco Prod. No.: C355)
Anti-Mouse CD71 (Transferrin Receptor)-Biotin (Leinco Prod. No.: C276)
Anti-Human CD71 (Transferrin Receptor) Purified Low Endotoxin Functional Formulation (Leinco Prod. No.: C371)
Anti-Human CD71 (Transferrin Receptor) Purified (Leinco Prod. No.: C163)
PN:
Anti-Mouse CD71 (Transferrin Receptor)-R-phycoerythrin (Leinco Prod. No.: C278)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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