Anti-Human CD71 – Purified in vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.C671
Clone
T56/14
Protein
CD71
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Transferrin Receptor
Isotype
Mouse
IgG1
Applications
FC
,
IHC FF
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C671-1.0 mg
1.0 mg
$198.00
In stock
Max:
Min: 1
Step: 1
C671-5.0 mg
5.0 mg
$398.00
In stock
Max:
Min: 1
Step: 1
C671-25 mg
25 mg
$1,150.00
In stock
Max:
Min: 1
Step: 1
C671-50 mg
50 mg
$1,825.00
In stock
Max:
Min: 1
Step: 1
C671-100 mg
100 mg
$2,500.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC
WB
Other Applications Reported In Literature ?
IHC FF
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone T56/14 recognizes human CD71.
Antigen Distribution
CD71 is expressed on most proliferating cells, activated lymphocytes, monocytes, macrophages, erythroid progenitors, and brain endothelium.
Background
CD71 is a 95 kD type II homodimeric transmembrane glycoprotein. The function of CD71 is believed to be primarily nutritional. It plays a role in the control of cellular proliferation through facilitation of iron uptake by way of ferrotransferrin binding and the recycling of apotransferrin to the cell surface. Additionally, transferrin receptor is required for erythropoiesis and proper neurological development, and it has also been suggested that a growth signal might be generated by the transferrin/transferrin receptor interaction. Current genetic analysis indicates that the structural genes for transferrin receptor and for a melanoma-associated antigen (p97), and perhaps transferrin itself, each map to a common chromosome in humans. These proteins exhibit primary sequence homology with transferrin and have the ability to bind ferric iron. Therefore, it is thought that genetic rearrangements in this iron transport region may be associated with malignant transformation. Hence, anti-CD71 mAbs are thought to have therapeutic potential in cases of human leukemia & lymphoma.

Antigen Details

Protein
Ligand/Receptor
Transferrin
PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. Pessin, JE. et al. (2003) J Biol Chem. 278(12):10683-90. Article Link
2. Trowbridge, SI. et al. (1981) Proc. Nat'l. Acad. Sci. 78:3039
3. Iacopetta, BJ. et al. (1983) J. Histochem. Cytochem. 31:336
Flow Cytometry
IHC
General Western Blot Protocol