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Anti-Human CD11b (CD11b)
Purified
(Mac-1 Integrin αMβ2)
Monoclonal Antibody
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Prod. No.:C133
Clone:44
Isotype:Mouse IgG1
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human CD11b (Clone 44) recognizes Human CD11b. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD11b recognizes the alpha subunit of the CD11b/CD18 complex, an alpha/Beta heterodimeric surface glycoprotein on human leukocytes (Mr 165-170 kDa).1,2 The CD11b/CD18 antigen belongs to the ß2 integrin family. Other names include Mac-1, Cr3, MO-1 and C3bi receptor. The CD11b molecule functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1.

Antigen Distribution:
The CD11b antigen is expressed on approximately 30% of human peripheral blood lymphocytes, most natural killer lymphocytes, circulating monocytes, granulocytes, a subset of T-cells and subsets of macrophages.1,2

Background:
Anti-CD11b may be used to study lymphocyte subsets in flow cytometry and in cell adhesion research (Mac-1). The antibody may be used to stain tissue sections which have been acetone fixed and frozen.

Host Species
Mouse
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This antibody is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Leukocyte Typing VI (1996) Oxford University Press
2. Myones, B. L. et al. (1988) J. Clin. Invest. 82:640
3. Malhotra, V. et al. (1986) Eur. J. Immunol. 16:1117
4. Leukocyte Typing II (1986) Oxford University Press: Code No. M104; III (1987) Oxford University Press: Code No. 202 and 238; IV (1989) Oxford University Press: Code No. M147
Related Products
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Anti-Human CD11b (Mac-1 Integrin αMβ2)-Fluorescein (FITC) (Leinco Prod. No.: C135)
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Anti-Mouse CD11b (Mac-1 αm chain)-Fluorescein (Leinco Prod. No.: C229)
Anti-Mouse CD11b (Mac-1 αm chain) Purified (Leinco Prod. No.: C227)
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Anti-Mouse CD11b (Mac-1 αm chain) Purified Low Endotoxin Functional Formulation (Leinco Prod. No.: C377)
Anti-Human CD11b (Mac-1 Integrin αMβ2) Purified Low Endotoxin Functional Formulation (Leinco Prod. No.: C368)
Anti-Human CD11b (Mac-1 Integrin αMβ2)-Biotin (Leinco Prod. No.: C134)
Anti-Human CD11b (Mac-1 Integrin αMβ2)-Allophycocyanin (APC) (Leinco Prod. No.: C307)
Anti-Mouse CD11b (Mac-1 αm chain)-Allophycocyanin (Leinco Prod. No.: C341)
Anti-Mouse CD11b (Mac-1 αm chain)-Biotin (Leinco Prod. No.: C228)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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