Anti-Human CD11b - DyLight® 755 - Leinco Technologies

Anti-Human CD11b – DyLight® 755

Product No.: C1725


Clone ICRF44 Target CD11b Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Mac-1 Integrin αMβ2 Isotype IgG1 Applications FC , IF Staining


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Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Purified Recombinant Human CD11b (>98%) Product Concentration 0.1 mg/ml Formulation This DyLight^® 755 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This DyLight^® 755 conjugate is stable when stored at 2-8°C. Do not freeze. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2828788 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this ICRF44 antibody for staining cells in flow cytometry is ≤ 1 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? IF Staining Additional Reported Applications For Relevant Conjugates ? B IF Microscopy IHC (Frozen) Costim For specific conjugates of this clone, review literature for suggested application details. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Clone ICRF44 recognizes the α subunit (CD11b) of the human CD11b/CD18 complex. Background LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or ß may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the ß2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/ß heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the ß2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis. Antigen Distribution CD11b is expressed on human peripheral blood lymphocytes, NK lymphocytes, monocytes, granulocytes, a subset of T-cells and macrophages. Ligand/Receptor ICAM-1(CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X PubMed CD11b NCBI Gene Bank ID 3684 UniProt.org Information on Uniprot.org Research Area Cell Adhesion . Cell Biology . Costimulatory Molecules . Immunology . Innate Immunity . Neuroscience . Neuroscience Cell Markers References & Citations 1. Leukocyte Typing VI (1996) Oxford University Press 2. Myones, B. L. et al. (1988) J. Clin. Invest. 82:640 3. Malhotra, V. et al. (1986) Eur. J. Immunol. 16:1117 4. Leukocyte Typing II (1986) Oxford University Press: Code No. M104 5. Leukocyte Typing III (1987) Oxford University Press: Code No. 202 and 238 6. Leukocyte Typing IV (1989) Oxford University Press: Code No. M147 View product citations for antibody C1725 on CiteAb Technical Protocols IF Staining Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human CD11b (>98%)

Product Concentration

0.1 mg/ml

Formulation

This DyLight^® 755 conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.

Storage and Handling

This DyLight^® 755 conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day 2-8°C

RRIDAB_2828788

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this ICRF44 antibody for staining cells in flow cytometry is ≤ 1 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.

Additional Applications Reported In Literature ?

IF Staining

Additional Reported Applications For Relevant Conjugates ?

B
IF Microscopy
IHC (Frozen)
Costim
For specific conjugates of this clone, review literature for suggested application details.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone ICRF44 recognizes the α subunit (CD11b) of the human CD11b/CD18 complex.

Background

LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or ß may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the ß2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/ß heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the ß2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.

Antigen Distribution

CD11b is expressed on human peripheral blood lymphocytes, NK lymphocytes, monocytes, granulocytes, a subset of T-cells and macrophages.

Ligand/Receptor

ICAM-1(CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X

PubMed

CD11b

NCBI Gene Bank ID

3684

UniProt.org

Information on Uniprot.org

Research Area

Cell Adhesion

Cell Biology

Costimulatory Molecules

Immunology

Innate Immunity

Neuroscience

Neuroscience Cell Markers

References & Citations

1. Leukocyte Typing VI (1996) Oxford University Press
2. Myones, B. L. et al. (1988) J. Clin. Invest. 82:640
3. Malhotra, V. et al. (1986) Eur. J. Immunol. 16:1117
4. Leukocyte Typing II (1986) Oxford University Press: Code No. M104
5. Leukocyte Typing III (1987) Oxford University Press: Code No. 202 and 238
6. Leukocyte Typing IV (1989) Oxford University Press: Code No. M147

View product citations for antibody C1725 on CiteAb

IF Staining

Certificate of Analysis

Request COA

Prod No.	Description
C1727	Anti-Human CD11b – PerCP
C1906	Anti-Mouse CD11b – PerCP
C1632	Anti-Mouse CD11b – DyLight® 650
C1719	Anti-Human CD11b – DyLight® 488
C1720	Anti-Human CD11b – DyLight® 550
C1721	Anti-Human CD11b – DyLight® 594
C1723	Anti-Human CD11b – DyLight® 650
C1725	Anti-Human CD11b – DyLight® 755

Formats Available

Prod No.	Description
C1727	Anti-Human CD11b – PerCP
C133	Anti-Human CD11b – Purified
C134	Anti-Human CD11b – Biotin
C135	Anti-Human CD11b – FITC
C1719	Anti-Human CD11b – DyLight® 488
C1720	Anti-Human CD11b – DyLight® 550
C1721	Anti-Human CD11b – DyLight® 594
C1723	Anti-Human CD11b – DyLight® 650
C1725	Anti-Human CD11b – DyLight® 755
C136	Anti-Human CD11b – PE
C510	Anti-Human CD11b – (PhenoCyler-Fusion (CODEX)® Ready)
C368	Anti-Human CD11b – Purified in vivo GOLD™ Functional Grade
C307	Anti-Human CD11b – APC
C668	Anti-Human CD11b – Purified in vivo PLATINUM™ Functional Grade

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-Human CD11b – DyLight® 755