Anti-Human CD11b – FITC
Anti-Human CD11b – FITC
Product No.: C135
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Clone ICRF44 Target CD11b Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Mac-1 Integrin αMβ2 Isotype IgG1 Applications FC |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Product Concentration 0.2 mg/ml Formulation This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze. Country of Origin USA Shipping Next Day Ambient Excitation Laser Blue Laser (490 nm) RRIDAB_2828420 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this ICRF44 antibody for staining cells in flow cytometry is ≤ 1 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. Additional Reported Applications For Relevant Conjugates ? B IF Microscopy IHC (Frozen) Costim For specific conjugates of this clone, review literature for suggested application details. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone ICRF44 recognizes the α subunit (CD11b) of the human CD11b/CD18 complex. Background LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or ß may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the ß2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/ß heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the ß2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis. Antigen Distribution CD11b is expressed on human peripheral blood lymphocytes, NK lymphocytes, monocytes, granulocytes, a subset of T-cells and macrophages. Ligand/Receptor ICAM-1(CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Adhesion . Cell Biology . Costimulatory Molecules . Immunology . Innate Immunity . Neuroscience . Neuroscience Cell Markers References & Citations1. Leukocyte Typing VI (1996) Oxford University Press
2. Myones, B. L. et al. (1988) J. Clin. Invest. 82:640
3. Malhotra, V. et al. (1986) Eur. J. Immunol. 16:1117
4. Leukocyte Typing II (1986) Oxford University Press: Code No. M104; III (1987) Oxford University Press: Code No. 202 and 238; IV (1989) Oxford University Press: Code No. M147 Technical ProtocolsCertificate of Analysis |
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