Anti-Human CD11b – (PhenoCyler-Fusion (CODEX)® Ready)

Anti-Human CD11b – (PhenoCyler-Fusion (CODEX)® Ready)

Product No.: C510

[product_table name="All Top" skus="C133"]

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Clone
ICRF44
Target
CD11b
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Mac-1 Integrin αMβ2
Isotype
IgG1
Applications
IHC FF
,
PhenoCycler®

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Product Size
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CODEX® Details

Validation Notes
Clone ICRF44 has been validated for use on CODEX® using human tonsil fresh frozen (FF) tissue.
Tissue Screened
Tonsil
Tissue Preparation
FF (Fresh Frozen)

Antibody and Reporter Details

Reactivity Species
Human
Host Species
Mouse
Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX® This CD11b (Clone ICRF44) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml.
RRID
AB_2893567
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone ICRF44 recognizes the α subunit (CD11b) of the human CD11b/CD18 complex.
Antigen Distribution
CD11b is expressed on human peripheral blood lymphocytes, NK lymphocytes, monocytes, granulocytes, a subset of T-cells and macrophages.
Background
LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or ß may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the ß2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/ß heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the ß2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.

Antigen Details

Protein
Ligand/Receptor
ICAM-1(CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X
PubMed
NCBI Gene Bank ID

References & Citations

1. Hitsuda, Yutaka et al. (1999) Yonago Acta medica 42:1–10 Article Link
2. Leukocyte Typing VI (1996) Oxford University Press
3. Myones, B. L. et al. (1988) J. Clin. Invest. 82:640
4. Malhotra, V. et al. (1986) Eur. J. Immunol. 16:1117
IHC
PhenoCycler®

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.