Anti-Human CD11b – DyLight® 650

Anti-Human CD11b – DyLight® 650

Product No.: C1723

[product_table name="All Top" skus="C1723"]

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Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Mac-1 Integrin αMβ2
IF Staining

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Antibody Details

Product Details

Reactive Species
Host Species
Purified Recombinant Human CD11b (>98%)
Product Concentration
0.2 mg/ml
This DyLight® 650 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This DyLight® 650 conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
Next Day 2-8°C
Excitation Laser
Red Laser (652 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this ICRF44 antibody for staining cells in flow cytometry is ≤ 1 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Additional Applications Reported In Literature ?
IF Staining
Additional Reported Applications For Relevant Conjugates ?
IF Microscopy
IHC (Frozen)
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.


Clone ICRF44 recognizes the α subunit (CD11b) of the human CD11b/CD18 complex.
LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or ß may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the ß2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/ß heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the ß2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.
Antigen Distribution
CD11b is expressed on human peripheral blood lymphocytes, NK lymphocytes, monocytes, granulocytes, a subset of T-cells and macrophages.

Antigen Details

ICAM-1(CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X
NCBI Gene Bank ID
Research Area
Cell Adhesion
Cell Biology
Costimulatory Molecules
Innate Immunity
Neuroscience Cell Markers

References & Citations

1. Leukocyte Typing VI (1996) Oxford University Press
2. Myones, B. L. et al. (1988) J. Clin. Invest. 82:640
3. Malhotra, V. et al. (1986) Eur. J. Immunol. 16:1117
4. Leukocyte Typing II (1986) Oxford University Press: Code No. M104
5. Leukocyte Typing III (1987) Oxford University Press: Code No. 202 and 238
6. Leukocyte Typing IV (1989) Oxford University Press: Code No. M147
Flow Cytometry
IF Staining

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.