Product Datasheet
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Goat F(ab')2 Anti-Mouse IgG (H&L)
Allophycocyanin (APC)
Secondary Antibody
Adsorbed Against: Hu., Bv., Hs.
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Size


Prod. No.:M101
Conc.:0.5 mg/ml
Pkg. Size:0.5 ml
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Goat F(ab')2 Anti-Mouse IgG (H&L) recognizes Mouse IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Reporter Molecule:
Allophycocyanin (APC) has a molecular weight of 104 kDa and is purified from green algae. APC is an intensely bright phycobiliprotein with a broad absorption profile that peaks at ~650 nm, and a fluorescence emission maximum of ~660 nm.

Preparation:
This F(ab')2 secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.
Host Species
Goat
Immunogen
Purified mouse IgG containing all isotypes.
Formulation
This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.
Reconstitution
The lyophilized secondary antibody Allophycocyanin (APC) conjugate should be reconstituted in 0.5 ml of deionized water. Gently vortex until the solution is clear. Centrifuge the conjugate if not completely clear and free of particulates.
Expiration Date
One year from date of reconstitution
Storage and Stability
This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted Allophycocyanin (APC) conjugate should be stored at 2-8°C. Do Not Freeze.
Applications and Suggested Working Dilutions
It is recommended that working dilutions be prepared fresh daily. A working dilution of 1:50 - 1:200 is recommended for most applications.
Country of Origin
USA
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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