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Anti-Human CD11c (CD11c)
R-phycoerythrin (R-PE)
(gp 150/95)
Monoclonal Antibody
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Prod. No.:C148
Clone:3.9
Isotype:Mouse IgG1
Conc.:0.1 mg/ml
Pkg. Size:50 µg, 100 µg, 200 µg
Storage:2-8°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human CD11c (Clone 3.9) recognizes Human CD11c. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD11c recognizes the (Mr 150 kDa) a-chain of the CD11c/CD18 complex. The antigen is the complement receptor type 4 (CR4). CD11c is an a/ß heterodimeric glycoprotein which belongs to the ß2 integrin family. It is also known as gp 150/95.

Antigen Distribution:
The CD11c antigen is present on monocytes and in lower density on granulocytes and NK cells. The antigen is strongly expressed in hairy cell leukemia and in some acute myeloid leukemias. Anti-Human CD11c (clone 3.9) stains macrophages in acetone-fixed frozen section of human lung, brain, colon, skin, spleen, thymus and tonsil.

Background:
Anti-Human CD11c may be used to stain monocytes and macrophages or hairy cell leukemia cells. This antibody can be used in studies of complement receptors. In malignant tissue, Anti-CD11c stains true histiocytic lymphomas and does not stain carcinomas or T- or B-lymphomas.

Reporter Molecule:
R-phycoerythrin (R-PE) has a molecular weight of 240 kDa and is purified from red algae. R-PE is an intensely bright phycobiliprotein with an absorption maxima at approximately 496, 546 and 565 nm, and a fluorescence emission maximum of ~578 nm.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Formulation
This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Stability
This R-PE conjugate is stable when stored at 2-8°C. Do not freeze.
Reported Applications
FC
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Leukocyte Typing V (1995) Oxford University Press
2. Leukocyte Typing IV (1989) Oxford University Press
3. Leukocyte Typing III (1987) Oxford University Press
4. Hogg, N. et al. (1986) Eur. J. Immunol. 16:240
5. Malhotra, V. et al. (1986) Eur. J. Immunol. 16:1117
Phycobilliproteins are protected under the following patents: (U.S. patents No. 4,520,110 and 4,542,104, European patent no. 76695, Canadian patent No. 1,179,942 and Australian patent No. 548,440).
Related Products
Anti-Human CD11c (gp 150/95)-Fluorescein (FITC) (Leinco Prod. No.: C147)
Anti-Human CD11c (gp 150/95) Purified (Leinco Prod. No.: C145)
PN:
Anti-Human CD11c (gp 150/95)-Biotin (Leinco Prod. No.: C146)
Anti-Human CD11c (gp 150/95) Purified Low Endotoxin Functional Formulation (Leinco Prod. No.: C369)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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