Anti-Human CD11c – Purified In vivo GOLD™ Functional Grade

Pricing & Details

Product No.C369
Clone
3.9
Protein
CD11c
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Integrin αX subunit, CR4, p150, ITGAX
Isotype
Mouse
IgG1 κ
Applications
B
,
CyTOF®
,
FA
,
FC
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C369-1.0 mg
1.0 mg
$95.00
In stock
Max:
Min: 1
Step: 1
C369-5.0 mg
5.0 mg
$298.00
In stock
Max:
Min: 1
Step: 1
C369-25 mg
25 mg
$800.00
In stock
Max:
Min: 1
Step: 1
C369-50 mg
50 mg
$1,325.00
In stock
Max:
Min: 1
Step: 1
C369-100 mg
100 mg
$1,998.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this 3.9 antibody for staining cells in flow cytometry is ≤ 2.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.>
WB The suggested concentration for this 3.9 antibody for use in western blotting is 1-10 μg/ml.
Other Applications Reported In Literature ?
CyTOF®
B
Additional Reported Applications For Relevant Conjugates ?
IHC (Frozen)
FA
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 3.9 recognizes the α-chain (CD11c) of the CD11c/CD18 complex. It is specific for the I domain of CD11c. Clone 3.9 binds the activated form of CD11c and partially blocks the binding of CD11c with ICAM-4.
Antigen Distribution
CD11c is primarily expressed on dendritic cells, NK cells, a subset of intestinal intraepithelial lymphocytes (IEL), and some activated T cells.
Background
LFA-1α (CD11a) and CD18 are the Integrin alpha-L and beta-2 chains respectively that combine to form LFA-1, a glycoprotein and a member of the Integrin family. Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3, ICAM4 and for F11R. LFA-1 participates in the immunological synapses between CD8+ T lymphocytes and antigen-presenting cells. The absence of LFA-1α or ß may induce LAD. The antigen contributes to natural killer cell cytotoxicity, and is involved in various immune phenomena such as leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. The CD11b/CD18 antigen is a heterodimeric surface glycoprotein on leukocytes and belongs to the ß2 integrin family. CD11b functions as a receptor for C3bi complement, clotting factor X, fibrinogen and ICAM-1. CD11c forms an α/ß heterodimeric glycoprotein (CD11c/CD18 complex) which belongs to the ß2 integrin family. The complex binds fibrinogen and reportedly serves as a receptor for iC3b and ICAM-1. During inflammatory responses, it mediates cell to cell interaction and is important in both monocyte adhesion and chemotaxis.

Antigen Details

Protein
Ligand/Receptor
CD54, fibrinogen, iC3b, ICAM-1, ICAM-4
Function
Adhesion, CTL killing
PubMed
NCBI Gene Bank ID
Research Area
Cell Adhesion
.
Cell Biology
.
Costimulatory Molecules
.
Immunology
.
Innate Immunity
.
Neuroscience
.
Neuroscience Cell Markers

References & Citations

1. Leukocyte Typing V (1995) Oxford University Press 2. Leukocyte Typing IV (1989) Oxford University Press 3. Leukocyte Typing III (1987) Oxford University Press 4. Hogg, N. et al. (1986) Eur. J. Immunol. 16:240 5. Malhotra, V. et al. (1986) Eur. J. Immunol. 16:1117
Flow Cytometry
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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