Recombinant Human Beta-Defensin-2
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BackgroundDefensins (alpha and beta) are cationic peptides with a broad spectrum of antimicrobial activity that comprise an important arm of the innate immune system. The alpha-defensins are distinguished from the beta-defensins by the pairing of their three disulfide bonds. To date, four human beta-defensins have been identified; BD-1, BD-2, BD-3 and BD-4. In addition to their direct antimicrobial activities, they are chemoattractant towards immature dendritic cells and memory T cells. The beta-defensin proteins are expressed as the C-terminal portion of precursors and are released by proteolytic cleavage of a signal sequence and, in the case of BD-1, a propeptide region. Beta-defensins contain a six-cysteine motif that forms three intra-molecular disulfide bonds. Human, rabbit and guinea-pig beta-defensins, as well as human beta-defensin-2 (hBD2), induce the activation and degranulation of mast cells, resulting in the release of histamine and prostaglandin D2. Protein DetailsPurity >95% by SDS-PAGE and HPLC Endotoxin Level <1.0 EU/µg as determined by the LAL method Biological Activity The biological activity of Human Beta-Defensin-2 is determined by its ability to chemoattract immature dendritic cells using a concentration range of 10.0-100.0 ng/ml. Amino Acid Sequence GIGDPVTCLK SGAICHPVFC PRRYKQIGTC GLPGTKCCKK P State of Matter Lyophilized Predicted Molecular Mass The molecular weight of Recombinant Human BD-2 is Mr 4.3 kDa. Storage and Stability The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day Ambient IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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