Recombinant Human BMP-6

Bone morphogenetic protein 6, also known as BMP6, is a human gene. The protein encoded by this gene is a a member of the TGFβ superfamily. Bone morphogenetic proteins are known for their ability to induce the growth of bone and cartilage. BMP6 is able to induce all osteogenic markers in mesenchymal stem cells. The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Based on its expression early in embryogenesis, the BMP encoded by this gene has a proposed role in early development. In addition, the fact that this BMP is closely related to BMP5 and BMP7 has led to speculation of possible bone inductive activity.

The biological activity of Human BMP-6 was determined by it ability to induce alkaline phosphatase expression by ATDC5 chodrogenic cells. The expected ED₅₀ for this effect is 0.05 - 0.15 μg/ml.

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Gln382

Solution

Recombinant Human BMP-6 is a homodimeric protein consisting of two 132 amino acid chains. The predicted molecular weight of each monomer is Mr 15 kDa. However due to glycosylation, the actual molecular weight of the doublet as observed by migration on SDS Page under reducing conditions is Mr 18 and 23 kDa.

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This recombinant protein is a 0.2 μm filtered solution in 50% acetonitrile (CH₃CN), 0.1% trifluoroacetic acid (TFA) containing 20 μg of rhBMP-6 at a concentration of 0.472 mg/ml.

Working aliquots of this recombinant protein solution are stable for up to twelve months at -20°C to -70°C in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2° - 8°C for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles.

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1. Vukicevic, S. et al. (2006) J Biol Chem. 281(35):25509-21.
2. Gruber, R. et al. (2006) J Periodontol. 77(4):699-706.
3. Guilak, F. et al. (2006) Arthritis Rheum. 54(4):1222-32.
4. Goldschmeding, R. et al. (2006) Diabetologia. 49(5):1039-48.
5. Makino, H. et al. (2006) Endocrinology. 147(6):2681-9.
6. Offenbacher, S. et al. (2006) Clin Oral Implants Res. 17(1):38-47.