Recombinant Human/Mouse FGF-8b
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BackgroundFibroblast growth factor-8 (FGF-8), also known as AIGF and HBGF, is a heparin binding growth factor belonging to the FGF family (1). Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation (2). Alternate splicing of FGF-8 mRNA creates eight secreted isoforms (a-h) in mice and four (a, b, e and f) in humans (3). FGF-8a expands the midbrain in transgenic mice, while FGF-8b transforms the midbrain into cerebellum. FGF-8 activates the “c” splice forms of receptors FGF R2, FGF R3 and FGF R4, with differential activity among the FGF-8 isoforms. Overexpression of FGF-8 has been shown to increase tumor growth and angiogenesis. FGF-8b shows the strongest receptor affinity and oncogenic transforming capacity, although isoforms a and e have been found in human tumors (4). The adult expression of FGF-8 is restricted to testes and ovaries. Protein DetailsPurity >97% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <0.01 EU/µg as determined by the LAL method Protein Accession No. Amino Acid Sequence mqvtvqssp nftqhvreqs lvtdqlsrrl irtyqlysrt sgkhvqvlan krinamaedg dpfaklivet dtfgsrvrvr gaetglyicm nkkgkliaks ngkgkdcvft eivlennyta lqnakyegwy maftrkgrpr kgsktrqhqr evhfmkrlpr ghhtteqslr feflnyppft rslrgsqrtw apepr
N-terminal Sequence Analysis Met State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Human/Mouse FGF-8b is Mr 22.5 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 23 kDa. Predicted Molecular Mass 22.5 Formulation This recombinant protein was lyophilized from a 0.2 μm filtered solution in MOPS, EDTA, Dithiothreitol (DTT), and sodium sulphate (Na2SO4). Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient NCBI Gene Bank References & Citations1. Gemel, J. et al. (1996) Genomics 35:253 2. Ruess, B. et al. (2003) Cell Tissue Res. 313:139 3. Tanaka, S. et al. (2001) Digest. Dis. Sci. 46:1016 4. Olsen, SK. et al. (2006) Genes Dev. 20:185 IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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