Recombinant Human BACE-1

BACE-1 is an aspartic protease and an integral membrane protein. BACE-1 is the peptidase predominantly responsible for cleavage of the amyloid precursor protein β site in the brain to generate the amyloid β peptide. Because the amyloid β peptide is a major component of amyloid plaques, BACE-1 has been implicated in the onset and/or progression of Alzheimer's disease. BACE-1 is expressed in a variety of human tissues. It is likely that this peptidase has functions in addition to its hydrolysis of the amyloid precursor protein. The peptidase activity of BACE-1 is optimal under mildly acidic conditions (pH 3.5 -5.5), consistent with its proposed function in an acidic intracellular compartment.

Histidine Tag

tqhgirlpl rsglggaplg lrlpretdee peepgrrgsf vemvdnlrgk sgqgyyvemt vgsppqtlni lvdtgssnfa vgaaphpflh ryyqrqlsst yrdlrkgvyv pytqgkwege lgtdlvsiph gpnvtvrani aaitesdkff ingsnwegil glayaeiarp ddslepffds lvkqthvpnl fslqlcgagf plnqsevlas vggsmiiggi dhslytgslw ytpirrewyy eviivrvein gqdlkmdcke ynydksivds gttnlrlpkk vfeaavksik aasstekfpd gfwlgeqlvc wqagttpwni fpvislylmg evtnqsfrit ilpqqylrpv edvatsqddc ykfaisqsst gtvmgavime gfyvvfdrar krigfavsac hvhdefrtaa vegpfvtldm edcgynipqt destlmtiay hhhhhhhhhh (pro) & etdee peepgrrgsf vemvdnlrgk sgqgyyvemt vgsppqtlni lvdtgssnfa vgaaphpflh ryyqrqlsst yrdlrkgvyv pytqgkwege lgtdlvsiph gpnvtvrani aaitesdkff ingsnwegil glayaeiarp ddslepffds lvkqthvpnl fslqlcgagf plnqsevlas vggsmiiggi dhslytgslw ytpirrewyy eviivrvein gqdlkmdcke ynydksivds gttnlrlpkk vfeaavksik aasstekfpd gfwlgeqlvc wqagttpwni fpvislylmg evtnqsfrit ilpqqylrpv edvatsqddc ykfaisqsst gtvmgavime gfyvvfdrar krigfavsac hvhdefrtaa vegpfvtldm edcgynipqt destlmtiay hhhhhhhhhh (mature)

Thr22 & Glu46

Lyophilized

The predicted molecular weight of Recombinant Human BACE-1 is Mr 50 (pro) and 47 (mature) kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 68-70 and 65-67 kDa.

50 (pro) and 47 (mature)

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

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