Recombinant Human Bcl-2-related Protein A1 (aa 1-152)

Bcl-2 related protein A1 (Bcl2A1) is a member of the Bcl-2 family of proteins that regulate outer mitochondrial membrane permeability (1). The proteins of this family act as anti- and pro-apoptotic regulators that are involved in a wide variety of cellular activities such as embryonic development, homeostasis and tumorigenesis. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such member is multi-domain antiapoptotic protein Bcl2A1, which is overexpressed in stomach and other cancers. Bcl2A1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation (2). It is upregulated in cisplatin-resistant human bladder tumor, suggesting that its expression may be important for cisplatin resistance and inhibition of apoptosis in cancer cells. At least two isoforms of Bcl2A1 are known to exist.

Histidine Tag

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Thr2

Solution

The molecular weight of Recombinant human BCL2A1 migrates as a monomeric 18 kDa polypeptide on SDS-reducing and non-reducing PAGE. Amino acid sequencing shows that the amino terminal methionine was removed during expression in E. coli.

This recombinant protein was 0.2 µm filtered and is supplied as a solution of 20 mM HEPES (pH 8.5), 0.1 M KCl and 20% glycerol.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one week or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day 2-8°C

1. Duriez, P. et al. (2000) J. Biol. Chem. 275:18099
2. Simmons, MJ. et al. (2007) Oncogene 27:1421