Recombinant Human CD22β/Siglec-2

CD22β, also known as Siglec-2, is a type 1 integral membrane glycoprotein belonging to the Ig family. It is a regulatory molecule that prevents the overactivation of the immune system and the development of autoimmune diseases. CD22β specifically recognizes sialic acid-containing glycans and is expressed on B cells (1). Both biochemical analysis on various B cells and studies using CD22β-deficient mice have demonstrated that CD22β negatively regulates signaling through the B cell antigen receptor (BCR), which plays a crucial role in response of B cells to antigen stimulation (2). CD22β does this by recruitment and activation of SH2-containing phosphatase 1 (SHP-1), which counteracts the phospharylation-mediated activation of signaling molecules by dephosphorylation, and reduces Ca2+ signaling by regulating the activity of the Ca2+ pump PMCA (3). Unlike other B cell markers, CD22β membrane expression is limited to the late differentiation stages comprised between mature B cells (CD22+) and plasma cells (CD22-), and may thus prove useful in phenotyping mature leukemias (4). CD22β is also strongly expressed in hairy cell leukemia.

Fc Fusion Protein

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Asp20

Lyophilized

The predicted molecular weight of Recombinant Human is Mr 101.9 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 122-127 kDa.

101.9

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Sgroi, D. et al. (1993) J. Biol. Chem. 268:7011
2. Poe, JC. et al. (2004) Nature Immunol. 5:1078
3. Tsubata, T. et al. (2001) J. Biol. Chem. 276:26648
4. Van Rossenberg, S. et al. (2001) J. Biol. Chem. 276:12967