Recombinant Human Growth Hormone

Growth hormone (GH), also known as somatotropin, is a hormone that stimulates growth, cell reproduction and regeneration. It is synthesized, stored, and secreted by the somatotroph cells within the lateral wings of the anterior pituitary gland and is stored in secretary granules. GH is released in a pulsatile manner by the combined actions of the hypothalamic hormones GHRH and SST as well as other factors (1). Several biological actions in the immune system are controlled by GH including enhancing thymopoiesis and T cell development, modulating cytokine production, enhancing B cell development and antibody production, priming neutrophils and monocytes for superoxide anion secretion, enhancing neutrophil adhesion and monocyte migration and anti-apoptotic action (2). In addition, GH can act directly on various cell types to induce lipolysis, lactation, amino acid uptake and protein synthesis (3-4).

The biological activity of Human GH was measured in a cell proliferation assay using the rat lymphoma, Nb2-11 (Gout, P.W. et al., 1980, Cancer Research 40:2433 - 2436). The expected ED₅₀ for this effect is typically 0.025 - 0.1 ng/mL.

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Met

Lyophilized

The predicted molecular weight of Recombinant Human GH is Mr 22 kDa.

22

This recombinant protein was 0.2 µm filtered and lyophilized from a sterile solution containing 10mM Tris, 75 mM NaCl pH 7.5.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Lin-Su, K. et al. (2002) Rev. Endocr. Metab. Disord. 3:313
2. Hattori, N. et al. (2009) Growth Horm. IGF Res. 19:187
3. Goffin, V. et al. (1996) Endocrine Rev. 17:385
4. Le Roith, D. et al. (2001) Endocrine Rev 22:53