Recombinant Human M-CSF R

Macrophage colony-stimulating factor (M-CSF, also termed CSF-1) receptor is an integral membrane tyrosine kinase, expressed in monocytes (macrophages and their progenitors) and drives growth and development of this blood cell lineage.^1,2,3 It also triggers growth and development of mononuclear phagocytes and placental trophoblasts.⁴ M-CSF receptor is present in osteoclasts both at mRNA and protein levels. When M-CSF ligand specifically binds to the M-CSF receptor on osteoclasts, it induces receptor dimerization, activation and autophosphorylation of cytoplasmic tyrosine residues⁵ M-CSF and its receptor present as candidate therapeutic targets in states of inflammatory bone erosion.⁶

Fc Fusion Protein

CAA27300

i pviepsvpel vvkpgatvtl rcvgngsvew dgpasphwtl ysdgsssils tnnatfqntg tyrctepgdp lggsaaihly vkdparpwnv laqevvvfed qdallpcllt dpvleagvsl vrvrgrplmr htnysfspwh gftihrakfi qsqdyqcsal mggrkvmsis irlkvqkvip gppaltlvpa elvrirgeaa qivcsassvd vnfdvflqhn ntklaipqqs dfhnnryqkv ltlnldqvdf qhagnyscva snvqgkhsts mffrvvesay lnlsseqnli qevtvgegln lkvmveaypg lqgfnwtylg pfsdhqpepk lanattkdty rhtftlslpr lkpseagrys flarnpggwr altfeltlry ppevsviwtf ingsgtllca asgypqpnvt wlqcsghtdr cdeaqvlqvw ddpypevlsq epfhkvtvqs lltvetlehn qtyecrahns vgsgswafip isagahthpp dediegrmdp kscdkthtcp pcpapellgg psvflfppkp kdtlmisrtp evtcvvvdvs hedpevkfnw yvdgvevhna ktkpreeqyn styrvvsvlt vlhqdwlngk eykckvsnka lpapiektis kakgqprepq vytlppsrde ltknqvsltc lvkgfypsdi avewesngqp ennykttppv ldsdgsffly skltvdksrw qqgnvfscsv mhealhnhyt qkslslspgk hhhhhh

Ile20

Lyophilized

The predicted molecular weight of Recombinant Human M-CSF R is Mr 81 kDa. However, as a result of glycosylation, the actual molecular weight of the monomer as observed by migration on SDS-PAGE is Mr 150 kDa.

81

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Stanley, ER. et al. (1978) Nature 274: 168
2. Byrne, PV. et al. (1981) J. Cell. Biol. 91: 848
3. Bourette, RP. et al. (2000) Growth Factors 17: 155
4. Stanley, ER. et al. (1996) Bone 18: 355
5. Novak, U. et al. (1996) Oncogene 13: 2607
6. Teitelbaum, SL. et al. (2005) J Clin Invest. 115(12):3418-27.