Recombinant Human NCAM-L1

The neural cell adhesion molecule L1 (NCAM-L1) is a type I membrane glycoprotein that is a member of the immunoglobulin superfamily adhesion molecule. It is critically involved in the development of the nervous system promoting neuronal migration, neuronal survival, neurite outgrowth, and myelination, as well as axon guidance, fasciculation, and regeneration.¹

The biological activity of Human NCAM-L1 was determined by the ability of the immobilized protein to support the adhesion of Neuro 2A cells, a mouse neuroblastoma cell line. When 5 x 10⁴ cells/well are added to human NCAM-L1/Fc coated plates (25 μg/ml with 100 μl/well), approximately 85 - 100% will adhere for 1 hour incubation at 37°C.

Fc Fusion Protein

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Ile20 & Arg864

Lyophilized

The predicted molecular weight of Recombinant Human NCAM-L1 is Mr 150.4 kDa (monomer) and 55.9 kDa (truncated). However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 210-225 kDa.

150.4 and 55.9

This recombinant protein was lyophilized from a 0.2 μm filtered solution in sodium chloride (NaCl) and tris-citrate.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Reiss, K. et al. (2005) Molec. Cellular Biol. 20: 9040