Recombinant Human Tie-1

Tyrosine kinase with immunoglobulin-like and EGF-like domains 1 (Tie-1) are novel endothelial cell tyrosine kinase receptors that are essential for vascular development and remodeling in the embryo.¹ They are expressed almost exclusively in endothelial cells.² Tie-1 also have a role in the regulatory pathways of early hematopoiesis and angiogenesis.³ It is upregulated in diseases such as atherosclerosis and rheumatoid arthritis.⁴

Fc Fusion Protein

avdltllan lrltdpqrff ltcvsgeaga grgsdawgpp lllekddriv rtppgpplrl arngshqvtl rgfskpsdlv gvfscvggag arrtrviyvh nspgahllpd kvthtvnkgd tavlsarvhk ekqtdviwks ngsyfytldw heaqdgrfll qlpnvqppss giysatylea splgsaffrl ivrgcgagrw gpgctkecpg clhggvchdh dgecvcppgf tgtrceqacr egrfgqscqe qcpgisgcrg ltfclpdpyg cscgsgwrgs qcqeacapgh fgadcrlqcq cqnggtcdrf sgcvcpsgwh gvhceksdri pqilnmasel efnletmpri ncaaagnpfp vrgsielrkp dgtvllstka ivepekttae fevprlvlad sgfwecrvst sggqdsrrfk vnvkvppvpl aaprlltkqs rqlvvsplvs fsgdgpistv rlhyrpqdst mdwstivvdp senvtlmnlr pktgysvrvq lsrpgeggeg awgpptlmtt dcpepllqpw legwhvegtd rlrvswslpl vpgplvgdgf llrlwdgtrg qerrenvssp qartalltgl tpgthyqldv qlyhctllgp asppahvllp psgppaprhl haqalsdsei qltwkhpeal pgpiskyvve vqvaggagdp lwidvdrpee tstiirglna strylfrmra siqglgdwsn tveestlgng lqaegpvqes raaeegldqq diegrmdpks cdkthtcppc papellggps vflfppkpkd tlmisrtpev tcvvvdvshe dpevkfnwyv dgvevhnakt kpreeqynst yrvvsvltvl hqdwlngkey kckvsnkalp apiektiska kgqprepqvy tlppsrdelt knqvsltclv kgfypsdiav ewesngqpen nykttppvld sdgsfflysk ltvdksrwqq gnvfscsvmh ealhnhytqk slslspgkhh hhhh

Ala22

Lyophilized

The predicted molecular weight of Recombinant Human Tie-1 is Mr 107 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 105-120 kDa.

107

This recombinant protein was 0.2 µm filtered and lyophilized from a sterile solution containing 20 mM Tris, 0.5 M NaCl and 10% Sucrose.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Vartikovski, L. et al. (1999) J Vasc Res. 36: 272
2. Peters, KG. et al. (2002) Mol Cell Biol. 22: 1704
3. Albita, M. et al. (2002) Cancer 94: 1517
4. Sukhatme, VP. et al. (2009) FEBS Lett.