Recombinant Mouse β-NGF

Nerve growth factor beta (NGFβ/beta-NGF) is a secreted protein of the neurotropin family and is important for the differentiation and survival of neurons and dermal keratinocytes (1). NGFβ is found in the hypothalamus, pituitary, thyroid gland, testes, epididymis, vascular smooth muscle cells, fibroblasts, mast cells and eosinophils. Glutamate, vitamin D3, IL-6, FGF basic, astrocyte specific IL-1, TNF alpha, PDGF and TGF beta up-regulate while GABAergic neuronal activity, glucocorticoids and Schwann cell-specific TGF beta down-regulate NFGβ. Signaling by NFGβ occurs through two receptors: TrKs and NGF receptor. It enhances the growth and differentation of B lymphocytes (2) and also has a possible role in allergy and tissue repair (3).

The biological activity of Mouse β-NGF was determined by in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1 (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323 - 334). The expected ED₅₀ for this effect is typically 0.3 - 1 ng/ml.

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Ser122

Lyophilized

The predicted molecular weight of Recombinant Mouse β-NGF is Mr 13.5 kDa.

13.5

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Schenck, K. et al. (2007) Eur. J. Oral Sci. 115:344
2. Otten, U. et al. (1989) Proc. Natl. Acad. Sci. (USA) 76:10059
3. Epplen, JT. et al. (2008) BMC Med. Genet. 9:107