Recombinant Rat EphA5

EPH receptor A5 (EPHA5) is a glycoprotein that is expressed by astrocytes and skeletal muscle. It provides guidance cues for retinal ganglion cells¹ and is essential for proper retinal axon guidance and topographic mapping in the mammalian visual system.² Ephrin A5 serves as a general inhibitor of axonal growth from limbic neurons.3 It also plays a role in motor axon outgrowth and pathfinding to the hindlimb.^4,5

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Ser58

Lyophilized

The predicted molecular weight of Recombinant Rat EphA5 is Mr 84 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 110 kDa.

84

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Davenport, RW. et al. (1998) J Neurosci. 18: 975
2. Frisen, J. et al. (1998) Neuron 20: 235
3. Gao, PP. et al. (1998) Proceedings of the National Acad of Sci. 95: 5329
4. Eberhart, J. et al. (2000) Developmental Neurosci. 22: 237
5. Eberhart, J. et al. (2004) J of Neurosci. 24: 1070