Recombinant Human BMP-7

TGF-β family members are key modulators of cell proliferation, differentiation, matrix synthesis, and apoptosis. As implied by their name, BMPs initiate, promote, and regulate the development, growth and remodeling of bone and cartilage. In addition to this role, BMPs are also involved in prenatal development and postnatal growth, remodeling and maintenance of a variety of other tissues and organs. BMP-7, also known as osteogenic protein-1 or OP-1, is a potent bone inducing agent, which in the presence of appropriate osteoconductive carrier (e.g. collagen sponge or synthetic hydroxyapatite) can be used in the treatment of bone defects. A bone-graft substitute, called OP-1TM implant, made of recombinant human BMP-7 associated with bovine bone-derived collagen, has recently been approved by the FDA as a device for treating critical-size bone fractures. The potential use of BMP-7 in dental reconstructive surgeries is currently under investigation. Recombinant human BMP-7 is a 28.8 kDa homodimeric glycoprotein consisting of two 116 amino acid subunits, which correspond to amino acid residues 316 to 431 of the full-length BMP-7 precursor.

BMP's are evolutionarily conseved acrross the animal species. For example human and mouse share 98% sequence identity of the mature protein. BMP-7 is expressed as a large precursor protein cleaved at the dibasic (RXXR) site to release the carboxy-terminal domain. Biologically active BMP-7 has been shown to exhib its cellular responses by the formation of hetero-oligomeric complexes of type I and II serine/threonine kinase receptors. BMP's 5,6,7,and 8 are in the same subgroup based on amino acid sequence similarity.

The biological activity of Human BMP-7 was determined by to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The expected ED₅₀ is typically 0.2-0.6 μg/ml.

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Ser293

Lyophilized

The predicted molecular weight of Recombinant Human BMP-7 is Mr 15.7 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 18-20 kDa.

15.7

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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1. Moreno, C. et al. (2008) Cancer Res. 2008 68(6): 1988–1996. PubMed