Recombinant Human BMP-7 (CHO Cell Expressed)

BMP-7 (osteogenic protein 1) belongs to the Bone-growth regulatory factors that are members of the transforming growth factor-beta (TGF-beta) superfamily of proteins. They are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. The active form can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins. N-TERMINAL---Human BMP-2 (Met 1 Arg 282) Human BMP-7 (Ser 293 Arg 431)---C-TERMINAL. The DNA sequence encoding the human BMP-2 signal peptide and propeptide (1~282 amino acid) fused to the human BMP-7 mature chain (293~431 amino acid) was expressed in a Chinese hamster ovary cell line. The mature recombinant BMP-7 generated by the proteolytic removal of the signal peptide and propetide contains 139 amino acid residues. The glycosylation of BMP-7 increases the molecular mass and the glycosylated proteins migrate as 25 ~ 40 kDa in SDS-PAGE under non-reducing conditions.

The biological activity of Human BMP-7 was determined by its ability to induce alkaline phosphatase production by mouse ATDC5 chondrogenic cells (Nakamura, K. et al., 1999, Exp. Cell Res. 250:351). The expected ED₅₀ is typically 0.2 - 0.6 μg/mL.

The DNA sequence encoding the human BMP-2 signal peptide and propeptide (amino acid residues 1 - 282 of human BMP-2) fused to the human BMP-7 mature chain (amino acid residues 293 - 431 of human BMP-7) cloned into a suitable mammalian expression vector and expressed in a Chinese hamster ovary cell line.

Lyophilized

The molecular weight of Recombinant Human BMP-7, due to glycosylation is approximately 30 - 40 kDa under non-reducing conditions and runs as an 18 - 20 kDa protein under reducing conditions.

This recombinant protein was lyophilized from a 0.2 μm filtered solution in 33% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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