Anti-Human IL-6 R – Biotin
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Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen Purified Recombinant Human IL-6 R (Accession # P08887) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830882 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human IL-6 R this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-6 R is 1 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1 - 0.4 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Anti-Human Interleukin 6 Soluble Receptor (IL-6 R) recognizes Human IL-6 R. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background IL-6 mediates its biological effects through the type I IL-6 receptor system that consists of two chains, IL-6R alpha and gp130. The IL-6R alpha chain is the binding component specific to IL-6; while the gp130 only transmits signals of IL-6 when bound to IL-6R alpha. The gp130 also can transmit signals from LIF, OSM, CNTF, IL-11 and CT-1 in conjunction with other receptor subunits. The low-affinity binding site for IL-6 is composed of IL-6R alpha alone. IL-6R alpha is expressed in a wide range of cells including T cells, fibroblasts and macrophages. Soluble IL-6R alpha which consists of only the extracellular domain of the IL-6R alpha chain, acts as an agonist of IL-6 activity at low concentrations. Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org Technical ProtocolsELISA Det  |
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