Anti-Human/Mouse BACE-1 Ectodomain
Anti-Human/Mouse BACE-1 Ectodomain
Product No.: B596
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Clone 137612 Target BACE-1 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1 Isotype IgG1 Applications CyTOF® , FC , IHC FFPE , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen NS0-Derived Recombinant Human BACE-1 Ectodomain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828266 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing BACE-1. A suggested method would be to stain cells expressing BACE-1 with 0.25 µg per 1-2.5 x 10 cells in a total staining volume of ≤200 µl followed by PN:M1189. Western Blotting: To detect Human BACE-1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human BACE-1 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 8 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human Beta-Site APP Cleaving Enzyme 1 (BACE-1) (Clone 137612) recognizes an epitope on Human BACE-1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. No cross-reactivity was observed with rhBACE-2, rmBACE-2, rhADAM8, rmADAM9, rmADAM10, rhADAM15, or rhTACE. Background BACE-1 is an aspartic protease and an integral membrane protein. BACE-1 is the peptidase predominantly responsible for cleavage of the amyloid precursor protein β site in the brain to generate the amyloid β peptide. Because the amyloid β peptide is a major component of amyloid plaques, BACE-1 has been implicated in the onset and/or progression of Alzheimer's disease. BACE-1 is expressed in a variety of human tissues. It is likely that this peptidase has functions in addition to its hydrolysis of the amyloid precursor protein. The peptidase activity of BACE-1 is optimal under mildly acidic conditions (pH 3.5 -5.5), consistent with its proposed function in an acidic intracellular compartment. Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org Technical ProtocolsCyTOF®  IHC FFPE  |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
