Anti-Human NCAM-L1 (Clone 84321)
Anti-Human NCAM-L1 (Clone 84321)
Product No.: N205
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Clone 84321 Target NCAM-L1 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names L1CAM, CAML1, CD171, HSAS, HSAS1, MASA, MIC5, S10, SPG1 Isotype IgG1 Applications WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen NS0-Derived Recombinant Human NCAM-L1 Extracellular Domain (Accession # CAA42508) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831438 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human NCAM-L1 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human NCAM-L1 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human Neural Cell Adhesion Molecule L1 (NCAM-L1) (Clone 84321) recognizes an epitope on Human NCAM-L1. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background The neural cell adhesion molecule L1 (NCAM-L1) is a type I membrane glycoprotein that is a member of the immunoglobulin superfamily adhesion molecule. It is critically involved in the development of the nervous system promoting neuronal migration, neuronal survival, neurite outgrowth, and myelination, as well as axon guidance, fasciculation, and regeneration.1 Antigen DetailsPubMed NCBI Gene Bank ID UniProt.org References & Citations1. Reiss, K. et al. (2005) Molec. Cellular Biol. 20: 9040 Technical Protocols |
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