Anti-Human NF-κB Activating Kinase (CT) (NAK)

PN:N151

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Human NF-κB Activating Kinase (NAK) recognizes an epitope near the C-terminus of Human and Mouse NAK. This polyclonal antibody was purified using affinity chromatography.

Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-κB is associated with IκB proteins in the cell cytoplasm, which inhibit NF-κB activity. Phosphorylation of I-κB by IκB kinase (IKK) complex leads to degradation of I-κB and activation of NF-κB. The IKK complex contains IKKα, IKKβ, and IKKγ. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-κB-activating kinase), and T2K.^1-3 NAK/TBK1 activates IKKβ through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-κB activation in response to growth factors.² NAK/TBK1 functions upstream of NIK and the IKK complex.^1,2 NAK/TBK1 is also critical in protecting embryonic liver from apoptosis.³

1. Pomerantz, JL. et al. (1999) EMBO J. 18(23):6694
2. Tojima, Y. et al. (2000) Nature 404(6779):778
3. Bonnard, M. et al. (2000) EMBO J. 19(18):4976